Local and systemic antibody responses to dextran-cholera toxin B subunit conjugates.

Infection and Immunity
C BergquistJ Holmgren

Abstract

This study was designed to test local and systemic immunity following mucosal immunization with a polysaccharide-protein conjugate. After preparing and characterizing dextran-cholera toxin B subunit (CTB) conjugates, we studied their immunogenicity in mice following systemic or mucosal immunizations. Dextran was chosen as a model polysaccharide antigen and conjugated via adipic acid dihydrazide and N-succinimidyl-3-(2-pyridyldithio)propionate to CTB. Mice were immunized either subcutaneously, intranasally, or perorally three times, and cholera toxin was used as an adjuvant for the mucosal immunizations. Three conjugates with different molecular weights for dextran (40,000 and 76,000) or varying dextran/CTB molar ratios were tested. Peroral immunizations with all conjugates evoked local immunoglobulin A (IgA) antibody responses against dextran in the small intestine, and intranasal immunizations did the same in the lung. Intranasal immunizations also elicited serum antibody titers that were significantly higher than or equal to those after subcutaneous immunizations. Intranasal immunizations evoked serum IgG antidextran titers which were dependent on the dextran/CTB molar ratio and inversely related to the local IgA response, wh...Continue Reading

References

Jan 1, 1978·Advances in Experimental Medicine and Biology·J R Cantey
Jan 1, 1977·Scandinavian Journal of Immunology·A M SvennerholmR J Rahimtoola
Jun 1, 1988·The Journal of Experimental Medicine·D Bessen, V A Fischetti
Mar 1, 1986·Journal of Medical Microbiology·A M Simoons-SmitD M MacLaren
Oct 10, 1985·Journal of Immunological Methods·A SuttonR Schneerson
Jan 1, 1972·The Journal of Clinical Investigation·P AndersonD H Smith
May 1, 1981·The Journal of Clinical Investigation·M E PichicheroR A Insel
Feb 1, 1982·The Journal of Pediatrics·D J PincusP F Wright
Mar 1, 1995·Infection and Immunity·M Quiding-JärbrinkC Czerkinsky
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Aug 1, 1982·Immunology Today·D E Mosier, B Subbarao

❮ Previous
Next ❯

Citations

Nov 24, 1999·Immunological Reviews·C CzerkinskyJ B Sun
May 20, 1998·Research in Immunology·N Lycke
May 4, 1999·Clinical and Diagnostic Laboratory Immunology·M FontanaR L Gregory
Oct 29, 1997·Journal of Virology·C M O'NealM E Conner

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.