Local anesthetics suppress nerve growth factor-mediated neurite outgrowth by inhibition of tyrosine kinase activity of TrkA

Anesthesia and Analgesia
Mayumi TakatoriMunetaka Hirose


Local anesthetics (LAs) suppress sympathetic sprouting, which correlates with neuropathic pain. However, the precise mechanism of the suppression is unknown. Nerve growth factor (NGF) contributes to the sympathetic sprouting, and NGF signaling starts with NGF-stimulated autophosphorylation of TrkA, which is a high affinity receptor of NGF. We examined the effects of lidocaine, bupivacaine, and procaine on NGF signaling under suppression of NGF-stimulated neurite outgrowth in PC12 cells, which is a cellular model of sympathetic sprouting. To investigate the effect of these LAs on NGF-mediated neurite outgrowth of PC12 cells, cells were incubated with 40, 400, and 4000 microM of each LA. The effect of LAs on NGF-stimulated TrkA activity was examined to analyze autophosphorylation of TrkA using immunoprecipitation and immunoblotting. Cytotoxic effects of LAs on PC12 cells were also assessed by lactate dehydrogenase release and by propidium iodide staining. Lidocaine (400 microM), bupivacaine (40 and 400 microM), or procaine (4000 microM) suppressed either neurite outgrowth or autophosphorylation significantly without cytotoxicity. The inhibition of NGF-stimulated tyrosine kinase activity of TrkA might be involved in the mechanisms...Continue Reading


Apr 13, 1995·European Journal of Pharmacology·J J WoodwardB R Martin
Mar 1, 1994·The Journal of Clinical Investigation·D ZachorI Ocrant
Apr 18, 1997·The Journal of Biological Chemistry·M E CunninghamL A Greene
Jun 23, 2000·Pain·Jianren Mao, Lucy L Chen
Sep 15, 2001·Nature·D Julius, A I Basbaum
Apr 27, 2002·British Journal of Pharmacology·Munetaka HiroseYoshifumi Tanaka
Aug 31, 2004·Anesthesia and Analgesia·Sachiko TodorokiKoji Sumikawa
Sep 17, 2004·Regional Anesthesia and Pain Medicine·Vasantha KumarAlix Mathieu
Oct 27, 2004·Anesthesiology·Michael E JohnsonGregory J Gores

❮ Previous
Next ❯


Jan 15, 2008·La Presse médicale·Luis Garcia-Larrea, Michel Magnin
Dec 2, 2014·Dental Clinics of North America·Mohanad Al-SabbaghMohd W Khalaf
Mar 26, 2011·Journal of Pharmaceutical Sciences·Dima AlukdaBi-Botti C Youan
Oct 10, 2015·Pain Practice : the Official Journal of World Institute of Pain·Munetaka HiroseEri Murata
Jun 11, 2015·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Cesar R Carcamo
Dec 23, 2006·Dental Clinics of North America·Christopher J Spencer, Henry A Gremillion
Mar 18, 2009·Neurourology and Urodynamics·Simone D GueriosDale E Bjorling
Jun 16, 2015·Current Psychiatry Reports·Eugene Lipov, Elspeth Cameron Ritchie
Jan 12, 2008·Journal of Pharmacological Sciences·Munetaka HiroseYoshifumi Tanaka
May 5, 2016·Autonomic Neuroscience : Basic & Clinical·Katia Puente de la Vega CostaLorenz Fischer
Apr 2, 2017·Journal of Molecular Neuroscience : MN·Eugene G LipovElspeth C Ritchie
Jul 11, 2007·Current Opinion in Anaesthesiology·James E Heavner
Nov 1, 2007·The Journal of Physiological Sciences : JPS·Tetsuro IsadaYoshifumi Tanaka
Jan 1, 2008·Anesthesia and Analgesia·Chiyo SatoAtsuhiro Sakamoto
Jul 23, 2011·Anesthesia and Analgesia·Hyung Seok YooChul Joong Lee
Jun 18, 2014·Menopause : the Journal of the North American Menopause Society·David R Walega, Pauline Maki
Jun 18, 2014·Menopause : the Journal of the North American Menopause Society·Eugene Lipov
Nov 29, 2016·Current Oral Health Reports·Annika RosénTie-Jun Shi
Jun 27, 2019·Journal of Neurophysiology·Fahad IqbalNaweed I Syed
Jul 11, 2007·Current Opinion in Anaesthesiology

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.