Local arginine supplementation results in sustained wound nitric oxide production and reductions in vascular endothelial growth factor expression and granulation tissue formation

The Journal of Surgical Research
Daithi HeffernanThomas R Howdieshell

Abstract

The goal of this work was to test the functional role of L-arginine in promotion of nitric oxide (NO) production and the vigorous granulation tissue formation characteristic of this wound model. Therapeutic use of supplemental arginine has been proposed as a safe and efficacious method to produce NO from nitric oxide synthase (NOS) and to produce proline and polyamines from arginase to improve wound healing. Although NO appears to be necessary to promote wound healing, the preferential metabolism of arginine to NO via NOS 2 may be detrimental if maintained beyond the initial days of healing. A ventral hernia, surgically created in the abdominal wall of 12 swine, was repaired with silicone sheeting and skin closure. Osmotic infusion pumps, inserted in remote subcutaneous pockets, continuously delivered saline (n = 6) or L-arginine (n = 6) into the wound environment. Granulation tissue thickness was determined by ultrasonography. Fluid was aspirated serially from the wound compartment for measurements of nitrite/nitrate (NOx), vascular endothelial growth factor (VEGF), transforming growth factor-beta1 (TGF-beta1), and amino acid concentrations. On day 14, the animals were sacrificed and the abdominal wall was harvested for immuno...Continue Reading

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Citations

May 4, 2012·BMC Complementary and Alternative Medicine·Laila M FaddahMuhammad Atteya
Jul 17, 2016·Acta Veterinaria Scandinavica·Kristiane BaringtonHenrik Elvang Jensen
Feb 6, 2019·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Ying LiShanglong Liu
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Aug 5, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yang ZhouJun Wu
Oct 28, 2008·Burns : Journal of the International Society for Burn Injuries·Willem M van der VeerFrank B Niessen

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