Local control of phosphatidylinositol 4-phosphate signaling in the Golgi apparatus by Vps74 and Sac1 phosphoinositide phosphatase.

Molecular Biology of the Cell
Christopher S WoodChristopher G Burd

Abstract

In the Golgi apparatus, lipid homeostasis pathways are coordinated with the biogenesis of cargo transport vesicles by phosphatidylinositol 4-kinases (PI4Ks) that produce phosphatidylinositol 4-phosphate (PtdIns4P), a signaling molecule that is recognized by downstream effector proteins. Quantitative analysis of the intra-Golgi distribution of a PtdIns4P reporter protein confirms that PtdIns4P is enriched on the trans-Golgi cisterna, but surprisingly, Vps74 (the orthologue of human GOLPH3), a PI4K effector required to maintain residence of a subset of Golgi proteins, is distributed with the opposite polarity, being most abundant on cis and medial cisternae. Vps74 binds directly to the catalytic domain of Sac1 (K(D) = 3.8 μM), the major PtdIns4P phosphatase in the cell, and PtdIns4P is elevated on medial Golgi cisternae in cells lacking Vps74 or Sac1, suggesting that Vps74 is a sensor of PtdIns4P level on medial Golgi cisternae that directs Sac1-mediated dephosphosphorylation of this pool of PtdIns4P. Consistent with the established role of Sac1 in the regulation of sphingolipid biosynthesis, complex sphingolipid homeostasis is perturbed in vps74Δ cells. Mutant cells lacking complex sphingolipid biosynthetic enzymes fail to prope...Continue Reading

References

Dec 1, 1989·The Journal of Cell Biology·A E ClevesV A Bankaitis
Aug 23, 1988·Biochemistry·K Simons, G van Meer
Sep 3, 1993·Science·M S Bretscher, S Munro
Dec 10, 1999·Nature Cell Biology·C Walch-Solimena, P Novick
Oct 7, 2003·The Journal of Biological Chemistry·Holger M RohdeGuido Boehmelt
Apr 27, 2004·Nature Cell Biology·Anna GodiM Antonietta De Matteis
Jul 24, 2004·The Journal of Biological Chemistry·Anjana Roy, Timothy P Levine
Dec 21, 2005·The Journal of Cell Biology·Thomas StrahlJeremy Thorner
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A SinghF Sherman
Apr 11, 2007·The Journal of Cell Biology·Todd I StrochlicChristopher G Burd
Apr 16, 2008·Developmental Cell·Karl R SchmitzKathryn M Ferguson
Apr 19, 2008·Science·Maureen E HillenmeyerGuri Giaever
Jul 19, 2008·Science·Linna TuDavid K Banfield
Nov 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jean-Baptiste MannevilleBruno Goud
Nov 3, 2009·Nucleic Acids Research·Judice L Y KohCharles Boone
Dec 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Françoise M RoelantsJeremy Thorner
Dec 23, 2009·The Journal of Cell Biology·Christopher S WoodChristopher G Burd
Jan 23, 2010·Science·Michael CostanzoCharles Boone
Feb 26, 2010·Nature·David K BreslowJonathan S Weissman
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Sumin HanAmy Chang
Jul 29, 2010·Glycobiology·Isaac CorbachoLuis M Hernández
Feb 2, 2011·Trends in Cell Biology·Todd R Graham, Christopher G Burd
Jul 19, 2011·Trends in Cell Biology·Felipe H Santiago-Tirado, Anthony Bretscher

❮ Previous
Next ❯

Citations

Jan 25, 2013·Molecular Biology of the Cell·Michelle M NgSeth J Field
Jan 18, 2013·ISRN Neurology·Toshihiro Masaki
Jan 8, 2014·Molecular Neurobiology·Ting LiZhiping Hu
Sep 3, 2014·The Journal of Cell Biology·Josse van GalenVivek Malhotra
Aug 13, 2014·The Journal of Cell Biology·Yiying CaiChristopher G Burd
Nov 7, 2015·Advances in Biological Regulation·Rossella VendittiMaria Antonietta De Matteis
Nov 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Richard J SucklingDavid J Owen
Dec 9, 2014·Insect Biochemistry and Molecular Biology·Zhaofei Li, Gary Blissard
May 29, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Maria A De MatteisGiovanni D'Angelo
May 16, 2014·Human Mutation·Liina NagirnajaMaris Laan
Sep 30, 2014·Biochimica Et Biophysica Acta·FoSheng Hsu, Yuxin Mao
Nov 14, 2013·Critical Reviews in Biochemistry and Molecular Biology·Julie Tan, Julie A Brill
Mar 14, 2013·FEMS Microbiology Reviews·Marizela DelicBrigitte Gasser
Oct 26, 2016·Chemistry and Physics of Lipids·Mark I McDermott, Carl J Mousley
Jul 6, 2016·Tissue & Cell·Riccardo RizzoAlberto Luini
Oct 7, 2018·FEBS Letters·Prasanna IyerDibyendu Bhattacharyya
Mar 30, 2018·G3 : Genes - Genomes - Genetics·Joana RodriguesDavid Lydall
Oct 29, 2015·EMBO Reports·Hazel F O'ConnorJon M Huibregtse
Jul 14, 2020·Frontiers in Cell and Developmental Biology·Subhrajit Banerjee, Patricia M Kane
Sep 13, 2020·Applied Microbiology and Biotechnology·Takao OhashiKaoru Takegawa
Jan 20, 2019·The Journal of Cell Biology·Rossella VendittiMaria Antonietta De Matteis
Jan 23, 2021·International Journal of Molecular Sciences·Katarzyna BiniędaDagmara Kabzińska
May 27, 2021·Archives of Biochemistry and Biophysics·Pablo Lujan, Felix Campelo
Sep 3, 2021·The Journal of Cell Biology·Lawrence G WelchSean Munro

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
affinity purification
two-hybrid
chip
bimolecular fluorescence complementation
interaction profiling
size exclusion chromatography
biosensor
deacylation

Software Mentioned

Volocity imaging
Prism
GraphPad
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemical Society Transactions
Hailan Piao, Peter Mayinger
Molecular Biology of the Cell
Michelle M NgSeth J Field
© 2021 Meta ULC. All rights reserved