Local delivery of bone morphogenetic protein-2 from near infrared-responsive hydrogels for bone tissue regeneration.

Biomaterials
Silvia Sanchez-CasanovaNuria Vilaboa

Abstract

Achievement of spatiotemporal control of growth factors production remains a main goal in tissue engineering. In the present work, we combined inducible transgene expression and near infrared (NIR)-responsive hydrogels technologies to develop a therapeutic platform for bone regeneration. A heat-activated and dimerizer-dependent transgene expression system was incorporated into mesenchymal stem cells to conditionally control the production of bone morphogenetic protein 2 (BMP-2). Genetically engineered cells were entrapped in hydrogels based on fibrin and plasmonic gold nanoparticles that transduced incident energy of an NIR laser into heat. In the presence of dimerizer, photoinduced mild hyperthermia induced the release of bioactive BMP-2 from NIR-responsive cell constructs. A critical size bone defect, created in calvaria of immunocompetent mice, was filled with NIR-responsive hydrogels entrapping cells that expressed BMP-2 under the control of the heat-activated and dimerizer-dependent gene circuit. In animals that were treated with dimerizer, NIR irradiation of implants induced BMP-2 production in the bone lesion. Induction of NIR-responsive cell constructs conditionally expressing BMP-2 in bone defects resulted in the forma...Continue Reading

Citations

May 12, 2020·Journal of Biological Engineering·Hongru LiZhiping Qi
Aug 12, 2020·Nanomedicine·Clara Escudero-Duch, Nuria Vilaboa
May 8, 2021·Biomacromolecules·Tianqi NieZhiyu He
May 25, 2021·Bioengineering & Translational Medicine·Sijing JiangJiacai He
Jun 3, 2021·International Journal of Molecular Sciences·Agnese GugliandoloFrancesca Diomede

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