Local delivery of fluorescent dye for fiber-optics confocal microscopy of the living heart

Frontiers in Physiology
Chao HuangFrank B Sachse

Abstract

Fiber-optics confocal microscopy (FCM) is an emerging imaging technology with various applications in basic research and clinical diagnosis. FCM allows for real-time in situ microscopy of tissue at sub-cellular scale. Recently FCM has been investigated for cardiac imaging, in particular, for discrimination of cardiac tissue during pediatric open-heart surgery. FCM relies on fluorescent dyes. The current clinical approach of dye delivery is based on systemic injection, which is associated with high dye consumption, and adverse clinical events. In this study, we investigated approaches for local dye delivery during FCM imaging based on dye carriers attached to the imaging probe. Using three-dimensional confocal microscopy, automated bench tests, and FCM imaging we quantitatively characterized dye release of carriers composed of open-pore foam only and foam loaded with agarose hydrogel. In addition, we compared local dye delivery with a model of systemic dye delivery in the isolated perfused rodent heart. We measured the signal-to-noise ratio (SNR) of images acquired in various regions of the heart. Our evaluations showed that foam-agarose dye carriers exhibited a prolonged dye release vs. foam-only carriers. Foam-agarose dye carr...Continue Reading

References

May 1, 1991·Optometry and Vision Science : Official Publication of the American Academy of Optometry·R P RutsteinJ B Eskridge
Jun 1, 1983·Thorax·R H AndersonA E Becker
Sep 1, 1994·Cell and Tissue Research·L J Andries, D L Brutsaert
Feb 1, 1996·Biophysical Journal·E M JohnsonW M Deen
Apr 28, 2004·Biophysical Journal·Nicolas Fatin-RougeJacques Buffle
Jul 14, 2005·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Ralf Kiesslich, Markus F Neurath
Jul 14, 2005·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Haruhiro InoueAkira Shiokawa
Jul 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Dhruba J BharaliMichal K Stachowiak
Feb 3, 2006·Clinical & Experimental Ophthalmology·Anthony S L KwanIan Constable
Aug 25, 2006·The Annals of Thoracic Surgery·Henrik Ø AndersenAndrew C Cook
Sep 30, 2006·EMBO Reports·Pierre VincentDanièle Paupardin-Tritsch
Jan 11, 2007·Journal of Microscopy·S Bolte, F P Cordelières
Nov 29, 2007·Current Opinion in Gastroenterology·Sharmila Anandasabapathy
Nov 5, 2008·Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie·Karen A Moosbrugger, Tom G Sheidow
Feb 14, 2009·The European Respiratory Journal·L ThibervilleG Bourg-Heckly
Dec 17, 2009·Alimentary Pharmacology & Therapeutics·M B WallaceR Kiesslich
Aug 5, 2011·Biology of the Cell·Ilse Hurbain, Martin Sachse
May 16, 2012·Journal of Interventional Gastroenterology·Muhammad W ShahidMichael B Wallace
Sep 29, 2012·The European Respiratory Journal·Mathieu SalaünLuc Thiberville
Apr 23, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Amaiak ChilingaryanGary G Martin

❮ Previous
Next ❯

Citations

Feb 23, 2020·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Aditya K KazaRobert Hitchcock

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
biopsy
optical microscopy
fluorescence imaging

Software Mentioned

MATLAB

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
Peter KellmanElliot R McVeigh
Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine
Logi VidarssonJohn M Pauly
Rinsho geka. Journal of clinical surgery
S SAKAKIBARA, H ORIHATA
© 2021 Meta ULC. All rights reserved