Local gene expression and immune responses of vaginal DNA vaccination using a needle-free injector

International Journal of Pharmaceutics
Takanori KanazawaHiroaki Okada

Abstract

The vaginal mucosa is the most common site of initiation of virus infections that are transmitted through heterosexual intercourse, including HIV and papillomavirus. Thus, in order to prevent or treat these infections, strong vaginal immunity is required as the first line of defense. In this study, to establish a less invasive, safe, convenient and effective immunization method, we examined the local (skin and vagina) gene transfection efficiency of a non-needle jet injector for daily insulin injection. In the skin experiment, the needle-free injector resulted in a marked increase in marker gene expression, compared to the conventional needle-syringe injection. In addition, intradermal DNA vaccination using the needle-free injector dramatically induced IFN-gamma and antibody systemic responses in mice. Furthermore, we investigated the applicability of the needle-free injector as a vaginal vaccination tool in rabbits. Vaginal gene expression using the needle-free injector was significantly greater than that using needle-syringe injection. Moreover, intravaginal vaccination by the needle-free injector promoted vaginal IgA secretion and IFN-gamma mRNA expression in the blood lymphocytes, to a degree significantly higher than that ...Continue Reading

Citations

Sep 16, 2011·Clinical and Vaccine Immunology : CVI·Guocai LiMingchun Ji
Sep 2, 2011·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hiroaki Okada
Nov 17, 2011·Vaccine·Vincent PavotStéphane Paul
Aug 28, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Teerawan RattanapakSarah Hook
Dec 1, 2020·Journal of Pharmaceutical Sciences·Hiroshi MiyazakiKazuhiro Terai
May 31, 2021·Advanced Drug Delivery Reviews·Zhi LuoJean-Christophe Leroux

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