Local heat activation of single myosins based on optical trapping of gold nanoparticles

Nano Letters
Mitsuhiro IwakiToshio Yanagida

Abstract

Myosin is a mechano-enzyme that hydrolyzes ATP in order to move unidirectionally along actin filaments. Here we show by single molecule imaging that myosin V motion can be activated by local heat. We constructed a dark-field microscopy that included optical tweezers to monitor 80 nm gold nanoparticles (GNP) bound to single myosin V molecules with nanometer and submillisecond accuracy. We observed 34 nm processive steps along actin filaments like those seen when using 200 nm polystyrene beads (PB) but dwell times (ATPase activity) that were 4.5 times faster. Further, by using DNA nanotechnology (DNA origami) and myosin V as a nanometric thermometer, the temperature gradient surrounding optically trapped GNP could be estimated with nanometer accuracy. We propose our single molecule measurement system should advance quantitative analysis of the thermal control of biological and artificial systems like nanoscale thermal ratchet motors.

References

Aug 17, 1999·Nature·A D MehtaR E Cheney
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·E M De La CruzH L Sweeney
Aug 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·M RiefJ A Spudich
Jan 28, 2003·Biophysical Journal·Erwin J G PetermanChristoph F Schmidt
Sep 26, 2003·Nature·Pierre-Damien CoureuxAnne Houdusse
Dec 5, 2003·Nature·David E Clapham
Mar 29, 2005·Biophysical Journal·Andrej Vilfan
Mar 17, 2006·Nature·Paul W K Rothemund
Jun 27, 2006·Nature·Jacob W J KerssemakersMarileen Dogterom
Jan 19, 2008·The Journal of Biological Chemistry·Yasuharu TakagiMihály Kovács
Jun 7, 2008·ACS Chemical Biology·Georgyi V LosKeith V Wood
Feb 4, 2009·The FEBS Journal·Yoshimitsu TakakuraTakeshi Yamamoto
May 22, 2009·Nature·Shawn M DouglasWilliam M Shih
Sep 20, 2011·Trends in Biotechnology·Georgeta Crivat, Justin W Taraska
Dec 21, 2011·Biochemical and Biophysical Research Communications·Kotaro OyamaShin'ichi Ishiwata
Jul 19, 2012·Nature Communications·Keisuke FujitaToshio Yanagida
Jun 15, 2011·Physical Review. B, Condensed Matter and Materials Physics·Alexander V SavinAlexey V Onufriev

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Citations

Nov 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Paden B RoderPeter J Pauzauskie
Jul 23, 2015·Biophysical Journal·Kotaro OyamaShin'ichi Ishiwata
Jul 28, 2015·Physical Chemistry Chemical Physics : PCCP·André HeberFrank Cichos
Aug 16, 2016·Advanced Materials·Xuezhe ZhouPeter J Pauzauskie
Jun 12, 2018·Chemistry, an Asian Journal·Matthew J CranePeter J Pauzauskie
Sep 1, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Majid SharifiMojtaba Falahati
Jun 13, 2017·Chemical Reviews·Fan HongHao Yan
Sep 15, 2017··Valentin A. MilichkoDmitry A. Zuev

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