Local innate responses and protective immunity after intradermal immunization with bovine viral diarrhea virus E2 protein formulated with a combination adjuvant in cattle

Vaccine
Sams M A SadatSylvia van Drunen Littel-van den Hurk

Abstract

Bovine viral diarrhea virus (BVDV) is one of the most serious pathogens in cattle. Recently, we developed a novel adjuvant platform (TriAdj) that includes a toll-like receptor 3 agonist, poly (I:C); an innate defense regulatory peptide; and water-soluble polymer, poly[di(sodiumcarboxylatoethylphenoxy)]-phosphazene (PCEP). To develop a needle-free intradermal (ID) subunit vaccine, the BVDV type-2 E2 protein was formulated with TriAdj, and immune protection was evaluated in calves against a BVDV-2 strain. Intradermal delivery of E2/TriAdj elicited robust virus neutralizing antibodies and cell-mediated immune responses including CD4+and CD8+T-cell responses. The development of CD8+T-cell responses in vaccinated calves indicates that TriAdj promotes cross-presentation. Upon challenge with virulent BVDV-2, the vaccinated calves showed no weight loss, leukopenia or virus shedding, and almost no temperature increase, in contrast to the control animals, which had severe clinical disease and shed virus for three to six days in nasal fluids and white blood cells. Intradermal vaccination was shown to attract various immune cell populations including dendritic cells, the most important antigen presenting cells. These data demonstrate that ...Continue Reading

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Citations

Dec 8, 2020·Frontiers in Immunology·Neha SangewarWaithaka Mwangi
Dec 8, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alexander K Andrianov, Robert Langer
Mar 26, 2021·Frontiers in Bioengineering and Biotechnology·Dylan J ChandGeorge K Mutwiri
Mar 26, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neha SangewarWaithaka Mwangi
May 11, 2021·Veterinary Immunology and Immunopathology·Kezia R FourieHeather L Wilson
Jun 1, 2021·Frontiers in Veterinary Science·Anwar A G Al-KubatiMaged Gomaa Hemida

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