Local origins impart conserved bone type-related differences in human osteoblast behaviour

European Cells & Materials
M ShahA A Pitsillides

Abstract

Osteogenic behaviour of osteoblasts from trabecular, cortical and subchondral bone were examined to determine any bone type-selective differences in samples from both osteoarthritic (OA) and osteoporotic (OP) patients. Cell growth, differentiation; alkaline phosphatase (TNAP) mRNA and activity, Runt-related transcription factor-2 (RUNX2), SP7-transcription factor (SP7), bone sialoprotein-II (BSP-II), osteocalcin/bone gamma-carboxyglutamate (BGLAP), osteoprotegerin (OPG, TNFRSF11B), receptor activator of nuclear factor-κβ ligand (RANKL, TNFSF11) mRNA levels and proangiogenic vascular endothelial growth factor-A (VEGF-A) mRNA and protein release were assessed in osteoblasts from paired humeral head samples from age-matched, human OA/OP (n = 5/4) patients. Initial outgrowth and increase in cell number were significantly faster (p < 0.01) in subchondral and cortical than trabecular osteoblasts, in OA and OP, and this bone type-related differences were conserved despite consistently faster growth in OA. RUNX2/SP7 levels and TNAP mRNA and protein activity were, however, greater in trabecular than subchondral and cortical osteoblasts in OA and OP. BSP-II levels were significantly greater in trabecular and lowest in cortical osteoblast...Continue Reading

Citations

Mar 2, 2018·International Journal of Environmental Research and Public Health·Caitlin L MaikawaKrystal J Godri Pollitt
May 16, 2017·The Analyst·Stephanie J SmithSumeet Mahajan
Apr 6, 2018·Frontiers in Bioengineering and Biotechnology·Behzad JavaheriAndrew A Pitsillides
Apr 13, 2019·BMC Veterinary Research·Richard L MeesonMichael Doube
Apr 6, 2019·Current Osteoporosis Reports·Lena Ingeborg Wolff, Christine Hartmann
Mar 20, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joji KusuyamaTetsuya Matsuguchi

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