Local radiotherapy of exposed murine small bowel: apoptosis and inflammation

BMC Surgery
Andrea PolistenaBengt Jeppsson


Preoperative radiotherapy of the pelvic abdomen presents with complications mostly affecting the small bowel. The aim of this study was to define the features of early radiation-induced injury on small bowel. 54 mice were divided into two groups (36 irradiated and 18 sham irradiated). Animals were placed on a special frame and (in the radiated group) the exteriorized segment of ileum was subjected to a single absorbed dose of 19 or 38 Gy radiation using 6 MV high energy photons. Specimens were collected for histology, immunohistochemistry (IHC) and ELISA analysis after 2, 24 and 48 hours. Venous blood was collected for systemic leucocyte count in a Burker chamber. Histology demonstrated progressive infiltration of inflammatory cells with cryptitis and increased apoptosis. MIP-2 (macrophage inflammatory protein) concentration was significantly increased in irradiated animals up to 48 hours. No significant differences were observed in IL-10 (interleukin) and TNF-alpha (tumour necrosis factor) levels. IHC with CD45 showed a significant increase at 2 hours of infiltrating leucocytes and lymphocytes after irradiation followed by progressive decrease with time. Caspase-3 expression increased significantly in a dose dependent trend in...Continue Reading


Oct 11, 1992·Journal of Leukocyte Biology·C M PayneD A Schultz
Aug 1, 1989·Journal of Surgical Oncology·V N AvizonisR D Noyes
Jun 1, 1988·International Journal of Radiation Oncology, Biology, Physics·F Ahmadu-SukaC E Whiteman
Oct 15, 1994·Cancer·J P DelaneyI Felemovicius
Jul 1, 1994·International Journal of Radiation Oncology, Biology, Physics·W F SindelarP A Johnstone
Jan 1, 1997·Cancer radiothérapie : journal de la Société française de radiothérapie oncologique·J B Dubois
Mar 3, 1999·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·M J EbleM Wannenmacher
Mar 29, 2000·Journal of Gastroenterology and Hepatology·A RamachandranK A Balasubramanian
Mar 22, 2001·International Journal of Radiation Biology·S L FreemanW K MacNaughton
Apr 6, 2001·International Journal of Cancer. Journal International Du Cancer·M MollàJ Panés
Nov 16, 2001·American Journal of Physiology. Heart and Circulatory Physiology·T O NossuliM L Entman
Sep 13, 2002·Brain Research. Molecular Brain Research·Stephanos KyrkanidesM Kerry O'Banion
Aug 21, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xin-Bing HanIsabelle G De Plaen


Apr 16, 2013·BioMed Research International·Agnès FrançoisMarc Benderitter
Jul 29, 2009·International Journal of Radiation Biology·Angelica FacoettiAndrea Ottolenghi
Jan 10, 2012·Experimental and Molecular Pathology·Silke CameronGiuliano Ramadori
Oct 16, 2013·Cancer Letters·Shankar SivaOlga A Martin
Oct 16, 2016·Journal of Immunotherapy·Jéssica Ribeiro GomesAntônio C Buzaid
Jan 13, 2018·Nutrition and Cancer·Sofia C M RietveldMarian A E de van der Schueren
Jun 15, 2018·Journal of Radiation Research·Sei Hwan YouChang Geol Lee
Apr 21, 2020·Plastic and Reconstructive Surgery. Global Open·Chatchai PruksapongKantang Satayasoontorn
Aug 12, 2016·Radiation Research·Maryam GoudarziHeng-Hong Li
Dec 4, 2020·International Journal of Molecular Sciences·Areumnuri KimSeung Sook Lee

Methods Mentioned

light microscopy

Related Concepts

Related Feeds


Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Caspases in Metabolic Diseases

Caspases, the family of cysteine proteases are involved in programmed cell death, but their role in metabolic diseases, inflammation and immunity has been of interested. Discover the latest research on caspases in metabolic diseases here.

Related Papers

International Journal of Radiation Oncology, Biology, Physics
Paul OkunieffJacob Finkelstein
Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer
Arsinoi XanthinakiEfstratios Patsouris
Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology
James W Denham, Martin Hauer-Jensen
© 2021 Meta ULC. All rights reserved