Mar 15, 2016

Local RhoA Activation Induces Cytokinetic Furrows Independent of Spindle Position and Cell Cycle Stage

BioRxiv : the Preprint Server for Biology
Elizabeth Wagner, Michael Glotzer

Abstract

Cytokinetic cleavage furrows assemble during anaphase at a site that is dictated by the position of the spindle. The GTPase RhoA promotes contractile ring assembly and furrow ingression during cytokinesis. While many factors that regulate RhoA during cytokinesis have been characterized, the spatiotemporal regulatory logic remains undefined. It is not known whether a local zone of RhoA activity is sufficient to induce furrow formation or whether the spindle modulates furrow assembly through other pathways. Similarly, it is not known whether the entire cortex is responsive to RhoA, nor whether contractile ring assembly is cell cycle regulated. Here, we have developed an optogenetic probe to gain tight spatial and temporal control of RhoA activity in mammalian cells and demonstrate that cytokinetic furrowing is primarily regulated at the level of RhoA activation. Light-mediated recruitment of a RhoGEF domain to the plasma membrane leads to rapid activation of RhoA, leading to assembly of cytokinetic furrows that partially ingress. Furthermore, furrow formation in response to RhoA activation is not spatially or temporally restricted. RhoA activation is sufficient to generate furrows at both the cell equator and at cell poles, in bo...Continue Reading

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Mentioned in this Paper

Biochemical Pathway
Spindle
Positioning Attribute
Cortex Bone Disorders
Adrenal Cortex Diseases
Interphase
Regulation of Biological Process
Spatial Distribution
Mitotic Metaphase
Mitotic Anaphase

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