PMID: 5257011Mar 1, 1969Paper

Local stimulation of growth in primary cultures of chick embryo fibroblasts

Proceedings of the National Academy of Sciences of the United States of America
T Gurney

Abstract

Confluent, slowly growing primary cultures of chick embryo fibroblasts were stimulated to grow more rapidly by making discontinuities in the monolayers by a "wounding" procedure. Growth was measured by mitotic index and by the fraction of cells synthesizing DNA as determined by autoradiography. Faster growth was confined to free cells at the edges of the discontinuities. Cells enclosed on all sides were not stimulated to renewed DNA synthesis, even when within 0.2 mm of the discontinuities. The time of appearance of increased rate of growth induced by wounding was measured and found to be similar to the time of a transient growth stimulation among enclosed cells induced by a change of medium. The increased rate of growth among free cells was not transient, however, and persisted as long as it was examined. To rule out possible artifacts incurred in wounding, a free edge was created by seeding trypsinized cells as a confluent sheet in a confined area of the tissue culture plate. Here also the growth rate of cells along the edge was much higher than in the confluent areas. It was concluded that interactions at short range between cells in culture can play an important role in regulating their growth.

References

Feb 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·D D PotterE S Lennox
May 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R W Holley, J A Kiernan
Dec 1, 1968·Journal of Cellular Physiology·L N Castor
Feb 1, 1969·The Journal of Cell Biology·J Yeh, H W Fisher
Oct 1, 1965·Experimental Cell Research·K Nilausen, H Green
Dec 1, 1965·Journal of Cellular Physiology·G J TodaroH Green
Nov 5, 1966·Nature·Y Kanno, W R Loewenstein
Jul 8, 1967·Nature·M G Stoker, H Rubin
Feb 3, 1967·Science·H W Fisher, J Yeh
Sep 1, 1964·The Journal of Cell Biology·W R LOEWENSTEIN, Y KANNO
Sep 1, 1964·The Journal of Cell Biology·J WIENERW R LOEWENSTEIN

❮ Previous
Next ❯

Citations

May 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·R Dulbecco, M G Stoker
Nov 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·H Rubin
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·G S MartinH Rubin
Dec 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·B M Sefton, H Rubin
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J B Baker, T Humphreys
Mar 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Rubin
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M M Nass
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G Lipkin, M E Knecht
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·H Rubin
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H Rubin, K Xu
Oct 1, 1987·The Journal of Cell Biology·R DermietzelJ P Revel
May 1, 1979·The Journal of Cell Biology·S C Selden, S M Schwartz
Apr 1, 1974·Experimental Cell Research·G R Martin, H Rubin
Feb 1, 1990·Experimental and Molecular Pathology·K Fischer-DzogaT Schaffner
Nov 1, 1973·Biochemical and Biophysical Research Communications·M MiyamotoT Onishi
Jun 15, 1976·Biochemical Pharmacology·G LipkinM E Knecht
Mar 1, 1981·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·S M SchwartzS C Selden
Jan 7, 1972·Nature·G S JohnsonI Pastan
Feb 17, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Harry Rubin
Apr 1, 1971·Journal of Cellular Physiology·M J Weber, H Rubin
Apr 1, 1972·Journal of Cellular Physiology·E Martz, M S Steinberg
Feb 1, 1972·Journal of Cellular Physiology·A D Glinos, R J Werrlein
Apr 1, 1975·Journal of Cellular Physiology·M C Canagaratna, P A Riley
Jun 1, 1972·Journal of Cellular Physiology·K A Ellem, S Mironescu
Jan 1, 1978·Journal of Cellular Physiology·A H Rubin, B Chu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.