Local Sustained Delivery of 25-Hydroxyvitamin D3 for Production of Antimicrobial Peptides

Pharmaceutical Research
J JiangJ Xie

Abstract

This study seeks to develop fiber membranes for local sustained delivery of 25-hydroxyvitamin D3 to induce the expression and secretion of LL-37 at or near the surgical site, which provides a novel therapeutic approach to minimize the risk of infections. 25-hydroxyvitamin D3 loaded poly(L-lactide) (PLA) and poly(ε-caprolactone) (PCL) fibers were produced by electrospinning. The morphology of obtained fibers was characterized using atomic force microscope (AFM) and scanning electron microscope (SEM). 25-hydroxyvitamin D3 releasing kinetics were quantified by enzyme-linked immunosorbent assay (ELISA) kit. The expression of cathelicidin (hCAP 18) and LL-37 was analyzed by immunofluorescence staining and ELISA kit. The antibacterial activity test was conducted by incubating pseudomonas aeruginosa in a monocytes' lysis solution. AFM images suggest that the surface of PCL fibers is smooth, however, the surface of PLA fibers is relatively rough, in particular, after encapsulation of 25-hydroxyvitamin D3. The duration of 25-hydroxyvitamin D3 release can last more than 4 weeks for all the tested samples. Plasma treatment can promote the release rate of 25-hydroxyvitamin D3. Human keratinocytes and monocytes express significantly higher ...Continue Reading

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Citations

May 19, 2017·Nanomedicine·Shixuan ChenJingwei Xie
Dec 7, 2017·Advanced Healthcare Materials·Shixuan ChenJingwei Xie
Mar 10, 2018·Journal of Periodontology·Zhanyi GaoHuanxin Meng
Sep 30, 2017·Nanomedicine·Shixuan ChenJingwei Xie
Nov 13, 2018·Expert Opinion on Drug Delivery·James R SmithDimitrios A Lamprou
Mar 28, 2021·International Journal of Biological Macromolecules·Mohammed Ahmad WsooNadirul Hasraf Mat Nayan

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