Localisation and expression of FoxO1 proteins in human gestational tissues

Placenta
Martha LappasMichael Permezel

Abstract

In non-gestational tissues, emerging data indicate that the FoxO1 family of Forkhead transcription factors play diverse roles in many cellular processes coordinating programs of gene expression that regulate apoptosis, oxidative stress resistance, and immune cell homeostasis. Successful outcome of human parturition rely on many of these processes, however there is no data available on FoxO1 proteins in human intrauterine tissues, nor their role in pregnancy complications such as pre-eclampsia. Thus the aim of this study was (i) to characterises the localisation and expression of FoxO1, acetylated (ac)-FoxO1 and phosphorylated (p)-FoxO1 in human placenta and fetal membranes obtained from term Caesarean sections (n=5); and (ii) to compare the expression of FoxO1 proteins in term placental samples from normal and pre-eclamptic pregnancies (n=5 per group). In placenta, weak FoxO1 staining was localised to the syncytiotrophoblast layer, whereas ac-FoxO1 and p-FoxO1 staining was mainly localised in the syncytiotrophoblasts and cytotrophoblasts. In fetal membranes, FoxO1, ac-FoxO1 and p-FoxO1 were localised to the trophoblast layer of the chorion, amnion epithelium and decidual cells. Quantitative RT-PCR (qRT-PCR) analysis showed a 6-...Continue Reading

Citations

Sep 29, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kenneth MaieseYan Chen Shang
Aug 19, 2010·Oxidative Medicine and Cellular Longevity·Kenneth MaieseYan Chen Shang
Mar 12, 2010·Journal of Clinical Pharmacology·Kenneth MaieseJinling Hou
Feb 14, 2015·Molecular and Cellular Endocrinology·Danalea V Skarra, Varykina G Thackray
Jun 19, 2009·Reproductive Sciences·Maria Irene Kokkinos
Aug 21, 2013·Reproduction, Fertility, and Development·Ratana LimMartha Lappas
Aug 14, 2018·MicroRNA·Georgios SkalisThomas Makris

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