PMID: 9178038Apr 1, 1997Paper

Localisation and expression of metallothionein immunoreactivity in the developing sheep brain

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
A F HollowayA K West

Abstract

Metallothioneins are small cysteine-rich proteins that bind heavy metals. In higher mammals there are complex families of metallothionein isoforms, which are well characterised at the DNA level but less so in terms of their cellular expression and function. In particular, little is known about the localisation of metallothionein in the developing mammalian brain. In this study using sheep fetuses, we have shown that metallothionein 1 and 2 isoform expression undergoes shifts in regional and cellular localisation during development of the brain. Metallothionein 1 and 2 expression is first detected by embryonic days E72 E73 (gestation is 150 days) at the mRNA level and the metallothionein protein is observed in cells of the proliferating ventricular zones. Subsequent expression is detected in radial glial cells, oligodendrocytes and astrocytes in several regions of the brain, most notably the cerebral cortex. In the adult brain, metallothionein is expressed in astrocytes but not in oligodendrocytes. Double-labelling immunohistochemistry using the glial fibrillary acidic protein (GFAP) an astrocyte marker, and metallothionein revealed that although there is an overlap in the profiles of the two proteins, there is no simple correla...Continue Reading

References

Jul 1, 1977·The British Journal of Nutrition·I BremnerB W Young
Feb 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N NishimuraC Tohyama
Jan 1, 1991·Methods in Enzymology·B Jasani, M E Elmes
May 1, 1989·Toxicology Letters·J Abel, N de Ruiter
Jun 1, 1988·European Journal of Biochemistry·M G Peterson, J F Mercer
Jul 1, 1984·Molecular and Cellular Biology·P F SearleR D Palmiter
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S A LewisN J Cowan
Apr 1, 1993·Journal of Neurocytology·K M DziegielewskaN R Saunders
Jul 1, 1994·Biological Signals·H G BlaauwgeersD Troost
Jul 1, 1994·Biological Signals·K SuzukiM Kimura
Jul 1, 1994·Journal of Dermatological Science·S ShimaokaH Ogawa
Aug 2, 1994·Biochimica Et Biophysica Acta·F A StennardA K West
Jun 1, 1994·Journal of Neuroscience Research·B H Juurlink
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·S ByravanA T Campagnoni
Jan 1, 1993·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·T Włostowski
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K T TamaiD J Thiele
Apr 1, 1993·The Biochemical Journal·L S Chubatsu, R Meneghini
Aug 1, 1993·Brain Research. Molecular Brain Research·H KobayashiS Tsuji

❮ Previous
Next ❯

Citations

May 1, 2004·Environmental Health and Preventive Medicine·Juan Hidalgo
Jan 7, 2004·Neuroscience·R S Chung, A K West
Jul 27, 2001·Brain Research Bulletin·J HidalgoM Vasák
May 22, 2003·Neurobiology of Disease·Javier CarrascoJuan Hidalgo
Jul 17, 2012·Chemical Reviews·Kasper P Kepp
Jul 23, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Adrian K WestRoger S Chung
Aug 28, 2021·International Journal of Molecular Sciences·Masato Asanuma, Ikuko Miyazaki

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.