Localization and Characterization of the Carbon Tetrachloride Transformation Activity of Pseudomonas sp. Strain KC.

Applied and Environmental Microbiology
M J DybasC S Criddle

Abstract

Previous research has established that Pseudomonas sp. strain KC rapidly transforms carbon tetrachloride (CT) to carbon dioxide (45 to 55%), a nonvolatile fraction (45 to 55%), and a cell-associated fraction ((equiv)5%) under denitrifying, iron-limited conditions. The present study provides additional characterization of the nonvolatile fraction, demonstrates that electron transfer plays a role in the transformation, and establishes the importance of both extracellular and intracellular factors. Experiments with (sup14)C-labeled CT indicate that more than one nonvolatile product is produced during CT transformation by strain KC. One of these products, accounting for about 20% of the [(sup14)C]CT transformed, was identified as formate on the basis of its elution time from an ion-exchange column, its boiling point, and its conversion to (sup14)CO(inf2) when incubated with formate dehydrogenase. Production of formate requires transfer of two electrons to the CT molecule. The role of electron transfer was also supported by experiments demonstrating that stationary-phase cells that do not transform CT can be stimulated to transform CT when supplemented with acetate (electron donor), nitrate (electron acceptor), or a protonophore (ca...Continue Reading

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Citations

Aug 30, 2001·Applied and Environmental Microbiology·J L SebatR L Crawford
Aug 11, 2010·FEMS Microbiology Ecology·Christian PennyFrançoise Bringel
Mar 13, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Timothy C Johnstone, Elizabeth M Nolan
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Sep 7, 2002·Environmental Science & Technology·Michael J DybasCraig S Criddle
Nov 16, 2002·Environmental Science & Technology·Jerry W GanderMichelle M Scherer

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