Localization and characterization of two novel genes encoding stereospecific dioxygenases catalyzing 2(2,4-dichlorophenoxy)propionate cleavage in Delftia acidovorans MC1

Applied and Environmental Microbiology
Kathleen M SchleinitzWolfgang Babel

Abstract

Two novel genes, rdpA and sdpA, encoding the enantiospecific alpha-ketoglutarate dependent dioxygenases catalyzing R,S-dichlorprop cleavage in Delftia acidovorans MC1 were identified. Significant similarities to other known genes were not detected, but their deduced amino acid sequences were similar to those of other alpha-ketoglutarate dioxygenases. RdpA showed 35% identity with TauD of Pseudomonas aeruginosa, and SdpA showed 37% identity with TfdA of Ralstonia eutropha JMP134. The functionally important amino acid sequence motif HX(D/E)X(23-26)(T/S)X(114-183)HX(10-13)R/K, which is highly conserved in group II alpha-ketoglutarate-dependent dioxygenases, was present in both dichlorprop-cleaving enzymes. Transposon mutagenesis of rdpA inactivated R-dichlorprop cleavage, indicating that it was a single-copy gene. Both rdpA and sdpA were located on the plasmid pMC1 that also carries the lower pathway genes. Sequencing of a 25.8-kb fragment showed that the dioxygenase genes were separated by a 13.6-kb region mainly comprising a Tn501-like transposon. Furthermore, two copies of a sequence similar to IS91-like elements were identified. Hybridization studies comparing the wild-type plasmid and that of the mutant unable to cleave dichl...Continue Reading

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Citations

Nov 13, 2008·Applied Microbiology and Biotechnology·Andreas Stolz
Jul 14, 2010·Antimicrobial Agents and Chemotherapy·Kathleen M SchleinitzSabine Kleinsteuber
Jun 29, 2010·Applied and Environmental Microbiology·Tina HölscherThomas Maskow
Sep 29, 2011·Applied and Environmental Microbiology·Diya SenEva M Top
May 27, 2005·Biochemical and Biophysical Research Communications·Gang-hua LangKiyotaka Miyashita
Nov 22, 2015·Applied and Environmental Microbiology·Louise FeldChristian Nyrop Albers
Jul 1, 2010·Journal of Agricultural and Food Chemistry·Jerry M Green, Micheal D K Owen

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