Localization and differential expression of the Krüppel-associated box zinc finger proteins 1 and 54 in early mouse development

DNA and Cell Biology
Maria AlbertsenKarin Lykke-Hartmann

Abstract

Upon fertilization, the zygotic genome is activated. To ensure the transcription of specific genes and avoid promiscuous gene expression, a chromatin-mediated repressive state is established. To characterize potential heterochromatin factors present during the first cleavage, two putative transcriptional repressors, zinc finger protein (ZFP1) and ZFP54, belonging to the Krüppel-associated box (KRAB) zinc finger family, were isolated. ZFP1 and ZFP54 contain an N-terminally located KRAB repressor domain followed by 8 and 12 repeats of Krüppel zinc-finger motifs, respectively. Reverse transcription (RT) and quantitative (q) PCR show that maternally contributed Zfp1 and Zfp54 mRNA are detected throughout preimplantation development. α-Amanitin-treated zygotes revealed that maternal Zfp1 and Zfp54 are fully degraded at the two-cell stage. Microinjections of in vitro-transcribed mRNA encoding a gfp-fused reporter gene into zygotes demonstrated the intracellular distribution of ZFP1-green fluorescent protein (GFP) and ZFP54-GFP colocalized with a DNA marker in the two-cell embryo. The KRAB domain was essential to colocalize with DNA, and deletion of the KRAB domain in ZFP1-GFP and ZFP54-GFP localized in nucleoli a...Continue Reading

References

Jan 14, 1977·Differentiation; Research in Biological Diversity·C M Warner, T F Hearn
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P H Crossley, P F Little
Oct 25, 1991·Nucleic Acids Research·M RosatiG Grimaldi
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E J BellefroidJ A Martial
Feb 1, 1991·Trends in Genetics : TIG·T Moore, D Haig
Oct 1, 1986·Developmental Biology·R A PedersenH Bałakier
Jan 1, 1985·The Journal of Experimental Zoology·G M KidderJ R McLachlin
Oct 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S Majumder, M L DePamphilis
Sep 22, 1995·The Journal of Biological Chemistry·J Y NothiasM L DePamphilis
Aug 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R M Schultz
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·J F MargolinF J Rauscher
Jan 1, 1993·Methods in Enzymology·M MirandaM L DePamphilis
Aug 15, 1996·Genes & Development·J R FriedmanF J Rauscher
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·S S KimJ V Bonventre
Mar 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·J Becker-FollmannO von Deimling
Jun 29, 1999·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M Shannon, L Stubbs
Feb 3, 2000·Nature Cell Biology·F Wianny, M Zernicka-Goetz
Jan 4, 2001·The International Journal of Biochemistry & Cell Biology·D T DangV W Yang
Mar 17, 2001·Nature Reviews. Genetics·W Reik, J Walter
Jun 13, 2001·Frontiers in Bioscience : a Journal and Virtual Library·K E Latham, R M Schultz
Aug 7, 2001·Frontiers in Bioscience : a Journal and Virtual Library·T P FlemingI Fesenko
Sep 22, 2001·Biology of Reproduction·J YuR M Schultz
Jan 14, 2003·Seminars in Cell & Developmental Biology·Anne Ferguson-SmithMaxine Tevendale
Jan 14, 2003·Seminars in Cell & Developmental Biology·Wendy DeanWolf Reik

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Citations

Oct 17, 2017·Frontiers in Cell and Developmental Biology·Trine StrandgaardKarin Lykke-Hartmann
May 14, 2020·Journal of Assisted Reproduction and Genetics·Mahboobeh AmoushahiKarin Lykke-Hartmann

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Datasets Mentioned

BETA
BC062970
BC080782

Methods Mentioned

BETA
PCR
light microscopy

Software Mentioned

boxshade
Excel
Pearl Primer
BLAST
LAS
Leica LAS
ClustalW
Leica
PROSITE

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