Localization and diversity of 185/333 proteins from the purple sea urchin--unexpected protein-size range and protein expression in a new coelomocyte type

Journal of Cell Science
Virginia BrocktonL Courtney Smith

Abstract

The current paradigm proposes that the innate immune systems of invertebrates are much more complex than previously thought. The highly diverse 185/333 gene family in the purple sea urchin encodes a family of closely related proteins of varying length and sequence composition. Subsets of small phagocytes and polygonal cells express 185/333 proteins with localization on the surface of the small phagocytes and within perinuclear vesicles in both cell types. In short-term cultures, coelomocytes form small aggregates that progress to syncytia that are thought to be equivalent to encapsulation in vivo. These aggregates were found to be enriched for 185/333-positive (185/333(+)) small phagocytes. In response to lipopolysaccharide challenge, coelomocytes transiently increased, including frequencies of both 185/333(+) and 185/333-negative (185/333(-)) small phagocytes and 185/333(+) polygonal cells. The 185/333 proteins were present in a broad array of sizes, most of which were larger than that predicted from the cDNAs. Recombinant 185/333 proteins expressed in bacteria and insect cells were also larger than expected, suggesting that the proteins dimerize and multimerize. The diversity of the 185/333 proteins, their expression in respo...Continue Reading

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Citations

Jul 9, 2009·PloS One·Francisco Ramírez-GómezJosé E García-Arrarás
Apr 25, 2013·PloS One·Audrey J MajeskeL Courtney Smith
Jan 17, 2012·FEBS Letters·Xianliang ZhaoYuanyou Li
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Dec 25, 2010·Developmental and Comparative Immunology·Julie GhoshL Courtney Smith
Nov 6, 2009·Developmental and Comparative Immunology·Julie GhoshL Courtney Smith
Dec 3, 2014·Developmental and Comparative Immunology·Chun LiKlara Stensvåg
Oct 19, 2016·Immunology and Cell Biology·Masayuki Hirano
Mar 1, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cheng Man LunL Courtney Smith
Oct 31, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Audrey J MajeskeL Courtney Smith
May 21, 2019·Frontiers in Immunology·Preethi GolcondaL Courtney Smith
Dec 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katherine M BuckleyL Courtney Smith
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nolwenn M DheillyDavid A Raftos
Jun 28, 2019·Frontiers in Immunology·Matan OrenL Courtney Smith
Sep 17, 2017·Developmental and Comparative Immunology·John DeFilippoGregory Beck
Apr 1, 2010·Disease Models & Mechanisms·L Courtney Smith
Nov 22, 2013·The Journal of Experimental Biology·Lage Cerenius, Kenneth Söderhäll

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