PMID: 20505367May 28, 2010

Localization and function of mSpindly during mouse oocyte meiotic maturation

Cell Cycle
Qing-Hua ZhangQing-Yuan Sun

Abstract

Spindly was first identified in Drosophila; its homologues are termed SPDL-1 in Caenorhabditis elegans and Hs Spindly/hSpindly in humans. In all species, Spindly and its homologues function by recruiting dynein to kinetochores and silencing SAC in mitosis of somatic cells. Depletion of Spindly causes an extensive metaphase arrest during somatic mitoses in Drosophila, C. elegans and humans. In Drosophila, Spindly is required for shedding of Rod and Mad2 from the kinetochores in metaphase; in C. elegans, SPDL-1 presides over the recruitment of dynein and MDF-1 to the kinetochores; in humans, Hs Spindly is required for recruiting both dynein and dynactin to kinetochores but it is dispensable for removal of checkpoint proteins from kinetochores. The present study was designed to investigate the localization and function of the Spindly homologue (mSpindly) during mouse oocyte meiotic maturation by immunofluorescent analysis, and by overexpression and knockdown of mSpindly. We found that mSpindly was typically localized to kinetochores when chromatin condensed into chromosomes after GVBD. In metaphase of both first meiosis and second meiosis, mSpindly was localized not only to kinetochores but also to the spindle poles. Overexpressio...Continue Reading

Citations

Nov 17, 2011·Human Reproduction Update·Shao-Chen Sun, Nam-Hyung Kim
Sep 3, 2011·Molecular Reproduction and Development·Heide Schatten, Qing-Yuan Sun
Jul 25, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Inbal Ben-EliezerNava Dekel
Jun 25, 2013·Genetics·Amber R KrauchunasMariana F Wolfner

Related Concepts

Spindly protein, mouse
M Phase, Meiotic
Metaphase
Microtubule-Associated Protein 3
Meiotic Spindle Apparatus
Oocytes
N-Myc Protein
Kinetochores
Cell Cycle Proteins
Chromosome Painting

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