PMID: 8471057Apr 1, 1993Paper

Localization and hormonal stimulation of phosphorylation sites in the LNCaP-cell androgen receptor

The Biochemical Journal
G G KuiperA O Brinkmann

Abstract

Phosphorylation of the androgen receptor in human prostate tumour cells (LNCaP) is increased by addition of androgens to intact cells. Double-label studies, using [35S]methionine incorporation into receptor protein, and [32P]P(i) to label metabolically receptor phosphorylation sites, have enabled us to determine the phosphate content, relative to receptor protein, of both nontransformed and transformed and androgen receptors generated in intact LNCaP cells. No net change in the phosphorylation of the intact 110 kDa steroid-binding component of the androgen-receptor complex was found upon transformation to the tight nuclear binding form in the intact cell. Partial proteolysis of androgen receptor protein metabolically labelled with [32P]P(i) and photolabelled with [3H]R1881 (methyltrienolone) revealed that phosphorylation occurs mainly in the N-terminal trans-activation domain, whereas no phosphorylation was detected in the steroid- and DNA-binding domains. The location of most (> 90%) of the hormonally regulated phosphorylation sites in the N-terminal trans-activation domain suggests a role of phosphorylation of the androgen receptor in transcription regulation.

Citations

Mar 31, 1995·The Journal of Biological Chemistry·G JensterA O Brinkmann
Mar 1, 1995·Journal of Cellular Biochemistry·B van SteenselR van Driel
Jun 7, 1996·The Journal of Biological Chemistry·G S TakimotoK B Horwitz
Aug 7, 2003·Cancer Investigation·Samit HirawatWilli Kreis
Oct 1, 1994·The Journal of Pediatric Endocrinology·A O Brinkmann
Apr 12, 2002·Endocrine Reviews·Cynthia A Heinlein, Chawnshang Chang
Aug 9, 2012·The Journal of Endocrinology·Kelly Coffey, Craig N Robson
Jul 9, 2004·The Biochemical Journal·Hao Yun WongAlbert O Brinkmann
Apr 1, 1996·Steroids·A BrinkmannJ Trapman
Jul 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·H E MacLeanJ D Zajac
Sep 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·T GaoM J McPhaul
Nov 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·J V Choate, J A Resko
Apr 28, 1995·Molecular and Cellular Endocrinology·P E de RuiterA O Brinkmann
Jul 1, 1995·Trends in Endocrinology and Metabolism : TEM·T R Brown
Apr 1, 1994·Molecular and Cellular Endocrinology·G G Kuiper, A O Brinkmann
Jan 16, 1999·Baillière's Clinical Endocrinology and Metabolism·O HiortE M Nitsche
Apr 1, 1994·Baillière's Clinical Endocrinology and Metabolism·M N PattersonI A Hughes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Molecular and Cellular Endocrinology
J H van LaarA O Brinkmann
Journal of Steroid Biochemistry
A O BrinkmannE Mulder
Lancet
Howard I ScherProstate Cancer Foundation/Department of Defense Prostate Cancer Clinical Trials Consortium
© 2021 Meta ULC. All rights reserved