Localization and induction by dehydration of ClC-K chloride channels in the rat kidney

The American Journal of Physiology
A VandewalleT J Jentsch

Abstract

We investigate the intrarenal expression of two recently cloned chloride channels, rClC-K1 and rClC-K2, by reverse transcriptase-polymerase chain reaction on single microdissected tubules from the rat kidney and by immunohistochemistry using a polyclonal antibody that recognizes both highly homologous channels. Both rClC-K1 and rClC-K2 mRNAs were detected in outer medullary late proximal tubules (S3), papillary ascending thin limbs (ATL), and outer medullary (MTAL) and cortical (CTAL) thick ascending limbs, distal tubules (DCT), and cortical, outer medullary, and inner medullary collecting ducts. Indirect immunofluorescence studies demonstrated that the rClC-K proteins were restricted to the basolateral membranes from ATL, DCT, and collecting ducts cells, whereas CTAL and MTAL exhibited a more diffuse basal staining. When rats were dehydrated, a condition which increased the expression of rClC-K1 in cortex and medulla, a weak cytoplasmic staining was found in late proximal tubule cells. Thus these results demonstrate that rat kidney ClC-K channels are predominantly located in the basolateral membranes from cells of the late segments of the renal tubule where most of chloride reabsorption takes place.

Citations

Feb 5, 2002·Annual Review of Physiology·Eric Delpire, David B Mount
Jul 29, 2004·American Journal of Physiology. Renal Physiology·Antoine NissantMartine Imbert-Teboul
Jan 13, 2006·American Journal of Physiology. Renal Physiology·Antoine NissantJacques Teulon
Mar 13, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Yi-Fang WangPung-Pung Hwang
Jul 28, 2020·American Journal of Physiology. Renal Physiology·Xiao-Tong SuDavid H Ellison
Jan 15, 2004·American Journal of Physiology. Renal Physiology·Wing Y LiAlan S L Yu
Mar 28, 2002·Physiological Reviews·Thomas J JentschAnselm A Zdebik
Jan 5, 2002·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Alain Vandewalle
Feb 16, 2005·Annual Review of Physiology·Thomas J JentschAnselm A Zdebik
Oct 25, 2007·Physiological Genomics·Panapat UawithyaMark A Knepper
May 20, 2011·American Journal of Physiology. Renal Physiology·Naohiro NomuraShinichi Uchida
Feb 22, 2001·Annual Review of Physiology·W B ReevesT E Andreoli
Feb 8, 2002·American Journal of Physiology. Renal Physiology·Raymond Mejia, James B Wade
Oct 2, 2003·American Journal of Physiology. Renal Physiology·Thomas L PannabeckerWilliam H Dantzler
May 11, 2006·American Journal of Physiology. Renal Physiology·Lawrence G Palmer, Gustavo Frindt
May 31, 2018·Physiological Reviews·Thomas J Jentsch, Michael Pusch
Nov 12, 2002·American Journal of Physiology. Renal Physiology·Olivier Devuyst, William B Guggino
Oct 29, 2000·American Journal of Physiology. Renal Physiology·S Uchida
Feb 9, 2000·American Journal of Physiology. Renal Physiology·T L PannabeckerW H Dantzler
Jul 26, 2002·Journal of the American Society of Nephrology : JASN·Katsuki KobayashiSei Sasaki
Jun 26, 2001·Journal of the American Society of Nephrology : JASN·Katsuki KobayashiFumiaki Marumo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Physiology. Renal Physiology
Wencui ZhengCharles S Wingo
Journal of the American Society of Nephrology : JASN
Dominique EladariRégine Chambrey
Proceedings of the National Academy of Sciences of the United States of America
S KieferleT J Jentsch
© 2021 Meta ULC. All rights reserved