Localization and interactions of Plasmodium falciparum SWIB/MDM2 homologues

Malaria Journal
Warren Antonio Vieira, T L Coetzer

Abstract

Malaria remains a global health problem and the majority of deaths are caused by Plasmodium falciparum parasites. Due to the rapid emergence of drug-resistant strains, novel avenues of research on the biology of the parasite are needed. The massive proliferation of asexual, intra-erythrocytic parasites every 48 h could kill the human host prior to transmission of slow-developing gametocytes to the mosquito vector. A self-induced P. falciparum programmed cell death mechanism has been hypothesized to maintain this balance between the parasite and its two hosts, but molecular participants of the cell death pathway in P. falciparum have not been characterized. Proteins with SWIB/MDM2 domains play a key role in metazoan programmed cell death and this study provides the first evaluation of two parasite SWIB/MDM2 homologues, PF3D7_0518200 (PfMDM2) and PF3D7_0611400 (PfSWIB). The function of these proteins was assessed by predicting their structural topology with the aid of bioinformatics and determining their location within live transgenic parasites, expressing green fluorescent protein-tagged PfMDM2 and PfSWIB under normal and elevated temperatures, which mimic fever and which are known to induce a programmed cell death response. Ad...Continue Reading

References

Aug 20, 1976·Science·W Trager, J B Jensen
Sep 1, 1996·Genes & Development·B R CairnsR D Kornberg
Nov 1, 1996·European Journal of Biochemistry·M G Claros, P Vincens
Feb 17, 1998·Transactions of the Royal Society of Tropical Medicine and Hygiene·S PicotP Ambroise-Thomas
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·R F WallerG I McFadden
Apr 9, 1999·Protein Engineering·B Rost
Mar 25, 2000·Molecular and Cellular Biology·I L de La SernaA N Imbalzano
Mar 29, 2001·Protein Science : a Publication of the Protein Society·C GeourjonG Deléage
Aug 4, 2001·Parasitology Research·H Y LongP G Kremsner
Feb 8, 2002·Nature·Louis H MillerOgobara K Doumbo
Feb 22, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hidemasa GotoMasaki Inagaki
May 23, 2002·Bioinformatics·Riccardo Bennett-LovseyKenji Mizuguchi
Sep 10, 2002·Bioinformatics·Christophe LambertEric Depiereux
Oct 9, 2002·Trends in Biochemical Sciences·Peter Tompa
Oct 9, 2002·Nature·Malcolm J GardnerBart Barrell
Feb 1, 2003·Science·Bernardo J FothGeoffrey I McFadden
Nov 6, 2003·Molecular and Biochemical Parasitology·Andreas BenderGisbert Schneider
Apr 22, 2004·Trends in Parasitology·Marcel Deponte, Katja Becker
Aug 4, 2004·Molecular and Biochemical Parasitology·Laurence FlorensYimin Wu
Oct 8, 2004·The Journal of Biological Chemistry·Qiyuan LiuJin Q Cheng
May 7, 2005·Acta Crystallographica. Section C, Crystal Structure Communications·Fang Jun HuoPin Yang
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Mar 3, 2007·Bioinformatics·Markus BrameierRobert M MacCallum
Nov 27, 2007·Trends in Parasitology·Roberto Lanzillotti, Theresa L Coetzer
Jan 24, 2008·Molecular and Cellular Biology·Sushma Shivaswamy, Vishwanath R Iyer
Feb 28, 2008·The Journal of Biological Chemistry·Jaehak OhRho H Seong
Feb 28, 2009·Nature Protocols·Lawrence A Kelley, Michael J E Sternberg
May 12, 2009·Nucleic Acids Research·Pascal BenkertTorsten Schwede
Jun 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Shunichi KosugiHiroshi Yanagawa

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Datasets Mentioned

BETA
AAB09030
ABV09038
ABD59092.1

Methods Mentioned

BETA
ubiquitination
transgenic
electrophoresis
phage display
PCR
fluorescence microscopy

Software Mentioned

PredSL
iPSORT Prediction
Image J
ESyPred3D Web Server
EMBOSS Needle
PyMOL Molecular Graphics System
BlastP
MitoProt II
PREDOTAR
Clustal Omega

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis