Localization and kinetics of protein kinase C-epsilon anchoring in cardiac myocytes

Biophysical Journal
Seth L RobiaJeffery W Walker

Abstract

Protein kinase C-epsilon (PKC-epsilon) plays a central role in cardiac cell signaling, but mechanisms of translocation and anchoring upon activation are poorly understood. Conventional PKC isoforms rely on a rapid Ca2+-mediated recruitment to cell membranes, but this mechanism cannot be employed by PKC-epsilon or other PKC isoforms lacking a Ca2+-binding domain. In this study, we used recombinant green fluorescent protein (GFP) fusion constructs and confocal microscopy to examine the localization, kinetics, and reversibility of PKC-epsilon anchoring in permeabilized rat cardiac myocytes. PKC-epsilon-GFP bound with a striated pattern that co-localized with alpha-actinin, a marker of the Z-line of the sarcomere. Binding required activation of PKC and occurred slowly but reversibly with apparent rate constants of k(on) = 4.6 +/- 1.2 x 10(3) M(-1) x s(-1) and k(off) = 1.4 +/- 0.5 x 10(-3) s(-1) (t1/2 = 8 min) as determined by fluorescence recovery after photobleaching and by perfusion experiments. A truncated construct composed of the N-terminal 144-amino-acid variable region of PKC-epsilon (epsilonV1-GFP), but not an analogous N-terminal domain of PKC-delta, mimicked the Z-line decoration and slow binding rate of the full-length e...Continue Reading

References

Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y OnoY Nishizuka
Mar 1, 1980·Journal of Muscle Research and Cell Motility·M Endo, M Iino
Feb 1, 1994·Circulation Research·V O Rybin, S F Steinberg
Feb 1, 1994·Experimental Cell Research·M H DisatnikD Mochly-Rosen
Apr 1, 1993·Cardiovascular Research·C S Lawson, J M Downey
Aug 1, 1995·Biochemical Society Transactions·S C KileyP J Parker
Apr 1, 1996·Current Opinion in Cell Biology·S Jaken
Oct 7, 1996·Biochimica Et Biophysica Acta·K YoshidaS Kawashiama
Jul 1, 1997·Circulation Research·Y PiJ W Walker
Nov 20, 1997·The Journal of Biological Chemistry·M CsukaiD Mochly-Rosen
Jul 3, 1999·The Journal of Biological Chemistry·M Medkova, W Cho
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·G W DornD Mochly-Rosen
Jun 9, 2000·Annual Review of Physiology·M V CohenJ M Downey

❮ Previous
Next ❯

Citations

Jan 18, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Derk FrankNorbert Frey
Dec 5, 2002·Cellular Signalling·Benoit Boivin, Bruce G Allen
May 11, 2010·Acta Pharmacologica Sinica·Shuang ZhangShi-jie Yang
Jan 25, 2011·The Journal of Biological Chemistry·Derk Frank, Norbert Frey
Apr 4, 2009·Physiological Reviews·Shuiping DaiJohannes W Hell
Oct 27, 2011·Journal of Biomedicine & Biotechnology·Ralph KnöllMax Lab
Jun 22, 2012·Biochemistry Research International·Javier T Granados-Riveron, J David Brook
May 6, 2014·BioMed Research International·Safa Abdul-GhaniM-Saadeh Suleiman
Mar 13, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Janet R ManningJo El J Schultz
Nov 21, 2007·Trends in Cardiovascular Medicine·Farah SheikhJu Chen
Dec 25, 2007·Journal of the American College of Surgeons·Neel R SodhaFrank W Sellke
Oct 3, 2015·Journal of Muscle Research and Cell Motility·Ying-Hsi LinBrenda Russell
May 15, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Klodiana Jani, Frieder Schöck
Apr 29, 2015·Cardiovascular Research·Emilie Dubois-DeruyFlorence Pinet
Oct 26, 2005·Current Biology : CB·Bernd MayerJuergen A Knoblich
Dec 9, 2003·The Journal of Biological Chemistry·Takuro ArimuraAkinori Kimura
Feb 6, 2004·The Journal of Biological Chemistry·Miriam C SouroujonDaria Mochly-Rosen
Jun 19, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Koji MiyazakiJames G Dobson
May 10, 2006·Molecular Carcinogenesis·Ajit K VermaHerbert Manoharan
Jun 21, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Seth L RobiaJeffery W Walker
Mar 11, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Ka Young ChungJeffery W Walker
Aug 21, 2013·The Journal of Biological Chemistry·Emily M SchulzDavid F Wieczorek
Dec 14, 2004·American Journal of Physiology. Cell Physiology·Nicole D SmallwoodCarole M Liedtke
May 28, 2005·Anesthesia and Analgesia·Lawrence A TurnerWai-Meng Kwok
Oct 3, 2003·American Journal of Physiology. Cell Physiology·Kerrie A BuhagiarHelge H Rasmussen
Nov 14, 2012·The Journal of Biological Chemistry·Emily M SchulzDavid F Wieczorek
Sep 16, 2003·The Journal of Biological Chemistry·Valentin G RobuJeffery W Walker
Nov 15, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Allen M Samarel
Jun 25, 2013·Journal of Muscle Research and Cell Motility·Emily M Schulz, David F Wieczorek
Apr 7, 2016·Journal of Receptor and Signal Transduction Research·Ya-Han HuangWei-Hsin Sun

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Related Papers

Journal of Molecular and Cellular Cardiology
Misuk Kang, Jeffery W Walker
Journal of Molecular and Cellular Cardiology
Leena KaikkonenRisto Kerkelä
Journal of Molecular and Cellular Cardiology
Caroline ChamberlandRobert Dumaine
Journal of Cardiovascular Medicine
Matthias ThielmannEva Kottenberg
© 2021 Meta ULC. All rights reserved