PMID: 7011405Mar 13, 1981Paper

Localization and partial purification of a neutral-active phospholipase A2 from BCG-induced rabbit alveolar macrophages

Biochimica Et Biophysica Acta
C Lanni, R Franson

Abstract

The localization and partial purification of a Ca2+-dependent, membrane associated phospholipase A2 (phosphatide 2-acylhydrolase, EC 3.1.1.4) from BCG-induced rabbit alveolar macrophages is described. Phospholipase A activity was determined using autoclaved Escherichia coli, the phospholipids of which were labelled in the 2-acyl position with [1-14C]oleate. Sonicated macrophages or granule preparations exhibited maximal phospholipase A2 activity at pH 7.0, with 5 mM Ca2+. Activity was quantitatively recovered in the pellet after centrifugation of homogenates at 100 000 x g, indicating that the enzyme is membrane-associated. At least two populations of macrophage granules were separated that contained phospholipase A2 activity. Plasma membranes enriched 15-fold with respect to alkaline phosphodiesterase I were devoid of phospholipase activity. The enzyme was purified 1278-fold in a yield of 34%, was active over a broad pH range, and was extremely sensitive to low concentrations of Ca2+. Mg2+ and Mn2+ would not substitute for Ca2+, 1 mM EDTA completely inhibited enzymatic activity. Absolute specificity for the 2-position was demonstrated using 1-[1-14C]stearyl-2-acyl 3-sn-glycerophosphorylethanolamine as substrate. Phospholipase ...Continue Reading

References

Dec 18, 1979·Biochimica Et Biophysica Acta·R L Jesse, R C Franson
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Citations

Feb 14, 1991·European Journal of Biochemistry·S P ChockE A Schmauder-Chock
Apr 1, 1985·Archives of Biochemistry and Biophysics·M I RossR J Ulevitch
Sep 15, 1982·Biochemical Pharmacology·K OhuchiS Tsurufuji
Jan 7, 1983·Biochimica Et Biophysica Acta·J K ThakkarR C Franson
Aug 4, 1999·Critical Reviews in Clinical Laboratory Sciences·E Kaiser
Mar 1, 1983·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section A, Pathology·T J NevalainenA K Suonpää
Apr 15, 1982·Biochimica Et Biophysica Acta·R M Hysmith, R C Franson
Apr 15, 1982·Biochimica Et Biophysica Acta·R C Franson, H Van den Bosch
Aug 18, 1982·Biochimica Et Biophysica Acta·J M De WinterH Van den Bosch
Nov 1, 1983·Biochimica Et Biophysica Acta·J K ThakkarR C Franson

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