PMID: 8945992Nov 1, 1996Paper

Localization and regulation by vitamin D of calcium transport proteins in rabbit cortical collecting system

The American Journal of Physiology
J van BaalRené J M Bindels

Abstract

The 1 alpha,25-dihydroxyvitamin D3 [1,25(OH)2D3]-induced expression of Na+/Ca2+ exchanger, Ca(2+)-adenosinetriphosphatase (Ca(2+)-ATPase), and calbindin-D28k was investigated in the rabbit distal nephron. Immunocytochemical studies in rabbit kidney sections revealed colocalization of the three Ca2+ transport proteins in the majority of cells in the distal nephron, including connecting tubules and cortical collecting ducts. Subsequently, rabbit connecting and cortical collecting tubule cells were immunodissected and cultured on permeable supports. Immunocytochemical analysis of the cultured cells by confocal microscopy revealed that Na+/Ca2+ exchanger and Ca(2+)-ATPase were present at the basolateral membrane, whereas calbindin-D28k was evenly distributed throughout the cytosol. Concomitant with an increase in Ca2+ transport, 1,25(OH)2D3 increased calbindin-D28k protein and RNA content two- to threefold, as determined by Northern and Western blotting. By contrast, neither Na+/Ca2+ exchanger nor Ca(2+)-ATPase RNA or protein content was noticeably altered. Our findings suggest that 1,25(OH)2D3 stimulation of transcellular Ca2+ transport in primary cultures of rabbit cortical collecting system cells involves an increase in the gene...Continue Reading

Citations

Mar 11, 2000·American Journal of Physiology. Renal Physiology·J G HoenderopR J Bindels
Jun 13, 2002·American Journal of Physiology. Renal Physiology·Clara E MagyarPeter A Friedman
Dec 25, 2004·Physiological Reviews·Joost G J HoenderopRené J M Bindels
May 18, 2006·American Journal of Physiology. Renal Physiology·Monique van AbelRené J M Bindels
Aug 12, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sylvie HuybersJoost G J Hoenderop
Oct 30, 2003·American Journal of Physiology. Renal Physiology·Sertac N Kip, Emanuel E Strehler
Apr 9, 2001·American Journal of Physiology. Renal Physiology·G RitchieG A Quamme
Jan 12, 2001·Physiological Reviews·L J DaiG A Quamme
Feb 5, 2002·Annual Review of Physiology·Joost G J HoenderopRene J M Bindels
Sep 17, 2005·Kidney International·Monique van AbelRené J M Bindels

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