PMID: 7236224Nov 15, 1980Paper

Localization and some properties of phosphate-dependent glutaminase in disrupted liver mitochondria

The Biochemical Journal
J D McGivanS K Joseph

Abstract

1. Glutaminase activity in frozen and thawed liver mitochondria was activated by NH4+, phosphate and HCO3-ions and also by ATP . 2. NH4+ and HCO3-ions decreased the requirement of the enzyme for phosphate. The activation by ATP was observed only in the presence of NH4+ or HCO3-ions. 3. In frozen-and-thawed mitochondria, the enzyme was loosely bound to the inner membrane, the Arrhenius plot showing a break at 23 degrees C. On sonication, glutaminase was detached from the membrane and the Arrhenius plot became linear. 4. The apparent Km for glutamine of the membrane-bound form was 6 mM, and that of the soluble form was 21 mM. 5. It is likely that the properties of glutaminase in the intact cell are dependent on the association of this enzyme with the mitochondrial membrane.

Citations

Nov 13, 1990·European Journal of Biochemistry·D HüssingerW Gerok
Mar 30, 1990·European Journal of Biochemistry·D HäussingerW Gerok
Feb 1, 1993·Journal of Cellular Physiology·W PfallerD Häussinger
Aug 25, 1980·FEBS Letters·P Lund
Nov 13, 2004·Biochimica Et Biophysica Acta·Michael R HaslettM E Brosnan
Jun 1, 1983·European Journal of Biochemistry·A J VerhoevenA J Meijer
Apr 15, 1985·European Journal of Biochemistry·J D McGivanN Bradford
Feb 2, 1987·European Journal of Biochemistry·H G HeiniD Mecke
Jun 16, 2015·The International Journal of Biochemistry & Cell Biology·Sara Rodríguez-EnríquezRafael Moreno-Sánchez
Jun 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Anila K MadirajuGerald I Shulman
Jan 24, 2017·Future Medicinal Chemistry·William P KattRichard A Cerione
Jan 1, 1995·Journal of Perinatal Medicine·C Bauer
Jul 16, 2014·Biomedit︠s︡inskai︠a︡ khimii︠a︡·P N Savilov
Mar 16, 2019·Journal of Clinical Medicine·Subbroto Kumar SahaKyoung Sik Park
Nov 20, 2019·International Journal of Biological Macromolecules·Tahany M MaharemYoussef A Said

Related Concepts

Enzyme Activation
Melting
Phosphate-Activated Glutaminase
D-Glutamine
Mitochondria, Liver
Orthophosphate
Sonication

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