Localization and structural analysis of a conserved pyruvylated epitope in Bacillus anthracis secondary cell wall polysaccharides and characterization of the galactose-deficient wall polysaccharide from avirulent B. anthracis CDC 684.

Glycobiology
L Scott ForsbergRussell W Carlson

Abstract

Bacillus anthracis CDC 684 is a naturally occurring, avirulent variant and close relative of the highly pathogenic B. anthracis Vollum. Bacillus anthracis CDC 684 contains both virulence plasmids, pXO1 and pXO2, yet is non-pathogenic in animal models, prompting closer scrutiny of the molecular basis of attenuation. We structurally characterized the secondary cell wall polysaccharide (SCWP) of B. anthracis CDC 684 (Ba684) using chemical and NMR spectroscopy analysis. The SCWP consists of a HexNAc trisaccharide backbone having identical structure as that of B. anthracis Pasteur, Sterne and Ames, →4)-β-d-ManpNAc-(1 → 4)-β-d-GlcpNAc-(1 → 6)-α-d-GlcpNAc-(1→. Remarkably, although the backbone is fully polymerized, the SCWP is the devoid of all galactosyl side residues, a feature which normally comprises 50% of the glycosyl residues on the highly galactosylated SCWPs from pathogenic strains. This observation highlights the role of defective wall assembly in virulence and indicates that polymerization occurs independently of galactose side residue attachment. Of particular interest, the polymerized Ba684 backbone retains the substoichiometric pyruvate acetal, O-acetate and amino group modifications found on SCWPs from normal B. anthrac...Continue Reading

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Feb 21, 2013·FEBS Letters·Ken-ichi YoritsuneKaoru Takegawa
Nov 12, 2014·Journal of Bacteriology·Megan Liszewski ZillaDominique Missiakas
Oct 30, 2014·PloS One·Mariana Claudia AllieviCarmen Sánchez-Rivas
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Nov 1, 2016·Journal of Bacteriology·So-Young OhDominique Missiakas
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Jan 23, 2021·International Journal of Molecular Sciences·Cordula StefanovićChristina Schäffer
Apr 9, 2020·Journal of Bioscience and Bioengineering·Takamasa FukunagaKaoru Takegawa

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