PMID: 391220Sep 1, 1979Paper

Localization by immunofluorescence and by light- and electron-microscopic immunoperoxidase techniques of Tamm-Horsfall glycoprotein in adult hamster kidney

The Biochemical Journal
K L SikriR D Marshall

Abstract

1. Tamm-Horsfall glycoprotein was isolated from hamster urine and antiserum against it was produced in rabbits. Immunoglobulin G was isolated from the antiserum. 2. Indirect methods of immunofluorescence staining were applied to kidney sections previously fixed by both perfusion and immersion methods. Tamm-Horsfall glycoprotein was identified associated with only the cells of the ascending limb of the loop of Henle and the distal convoluted tubule. Maculae densae were free of the glycoprotein. 3. Indirect immunoperoxidase procedures with light microscopy were applied to kidney sections. The results extended those found by immunofluorescence by showing that the glycoprotein is largely associated with the plasma membrane of the cells. Macula densa cells were shown to be free of the glycoprotein, although the luminal surface of the remaining cells in the transverse section of the nephron at that region was shown to contain it. 4. A variety of immuno-electron-microscopic techniques were applied to sections previously fixed in a number of ways. Providing periodate/lysine/paraformaldehyde was used as the fixative, the glycoprotein was often seen to be present not only on the luminal surface of the cells of the thick ascending limb of...Continue Reading

Citations

Nov 1, 1985·The Histochemical Journal·S KumarA W Asscher
Jan 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J Davies
Nov 16, 2011·Human Molecular Genetics·Nicole GrögerThomas Boettger
Oct 13, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cheng-Han WuChia-Li Yu
May 1, 1988·Immunological Investigations·J A MortonR I Vanhegan
Jan 13, 1999·Glycobiology·J J van RooijenJ F Vliegenthart
Jul 1, 1997·Clinical and Experimental Pharmacology & Physiology·L Barajas
Mar 20, 2003·American Journal of Physiology. Renal Physiology·Peter K StricklettDonald E Kohan
Apr 21, 2006·Glycoconjugate Journal·Franca Serafini-CessiDaniela Cavallone
Jan 25, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tsai-Hung WuChang-Youh Tsai
Jan 1, 1986·Virchows Archiv. A, Pathological Anatomy and Histopathology·G Zanetti
Dec 1, 1985·European Journal of Clinical Microbiology·P M Miniter, V T Andriole
Jan 8, 2005·Nephron. Physiology·Viswanathan PragasamPalaninathan Varalakshmi
Mar 1, 2009·Proteomics. Clinical Applications·Wei SunYouhe Gao
Nov 15, 1984·Carbohydrate Research·J WilliamsJ F Vliegenthart
Apr 9, 2002·Clinical Biochemistry·María C RomeroAlcira Nesse
Feb 1, 1997·Clinical Biochemistry·M C RomeroA B Nesse
May 20, 1999·American Journal of Human Genetics·F ScolariG Casari
Aug 1, 2009·EcoSal Plus·Harry L T MobleyErin C Hagan
Oct 2, 2003·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Franca Serafini-CessiDaniela Cavallone
May 1, 1981·Developmental Biology·P EkblomL Saxén
Dec 10, 1999·The Urologic Clinics of North America·D E Neal
Feb 1, 1990·Kidney International·J K HortonJ D Williams
Dec 1, 1990·Kidney International·T IshidateJ R Hoyer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.