Localization, cloning, and expression of genetic determinants for bacteriophage resistance (Hsp) from the conjugative plasmid pTR2030.

Applied and Environmental Microbiology
Colin HillT R Klaenhammer

Abstract

Genetic determinants for a bacteriophage resistance mechanism (Hsp+) encoded by plasmid pTR2030 (46.2 kilobases [kb]) were localized by mapping an 11.5-kb deletion that accompanied the transition of Lactococcus lactis LMA12-4 transconjugants (M. E. Sanders, P. J. Leonard, W. D. Sing, and T. R. Klaenhammer, Appl. Environ. Microbiol. 52:1001-1007, 1986) from phage resistance to phage sensitivity. The deleted 34.7-kb replicon (pTR2023, Hsp-) retained its conjugative ability, demonstrating that the phage resistance and conjugal transfer determinants were genetically distinct. The Hsp region of pTT2030, which was contained within a 13.6-kb BglII fragment, was cloned into the BamHI site of bacteriophage lambda EMBL3, and Hsp was subcloned into the Escherichia coli-Streptococcus shuttle vector pSA3. The recombinant plasmids pTK6 and pTK9 were recovered in E. coli HB101 and contained a 13.6-kb insert in opposite orientations. L. Lactis MG1363 transformants carrying pTK6 or pTK9 exhibited a significant reduction in plaque size, in addition to a slight reduction in the efficiency of plaquing for both prolate and small isometric phages. Phenotypic reactions observed for the recombinant plasmids suggest that pTR2030-encoded Hsp acts simila...Continue Reading

References

Feb 1, 1988·Journal of Dairy Science·B R FrosethL L McKay
Jan 1, 1985·Applied and Environmental Microbiology·M L Dao, J J Ferretti
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Aug 1, 1984·Applied and Environmental Microbiology·J K Kondo, L L McKay
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Sep 1, 1983·Applied and Environmental Microbiology·D G Anderson, L L McKay
Jul 1, 1980·Applied and Environmental Microbiology·L L McKayP M Walsh
May 1, 1982·Applied and Environmental Microbiology·J K Kondo, L L McKay
Jun 1, 1986·Applied and Environmental Microbiology·W D Sing, T R Klaenhammer
Jun 1, 1975·Applied Microbiology·B E Terzaghi, W E Sandine

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Citations

Feb 14, 2006·FEMS Microbiology Reviews·Susan MillsR Paul Ross
Apr 16, 1998·Molecular Microbiology·D J O'Sullivan, T R Klaenhammer
Jan 1, 1993·Journal of Dairy Science·D A Romero, T R Klaenhammer
Dec 1, 1995·Molecular Biotechnology·P K Dinsmore, T R Klaenhammer
Sep 12, 2002·International Journal of Food Microbiology·Egon Bech Hansen
Jan 1, 1991·Applied and Environmental Microbiology·C HillT R Klaenhammer
Feb 1, 1993·Applied and Environmental Microbiology·W D Sing, T R Klaenhammer
Jun 1, 1994·Applied and Environmental Microbiology·S MoineauT R Klaenhammer

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