Localization, identification, and action of galanin in the frog adrenal gland

Endocrinology
S GasmanCatherine Delarue

Abstract

The distribution of galanin-like immunoreactivity was studied in the adrenal gland of the frog Rana ridibunda using the indirect immunofluorescence technique. A dense network of varicose fibers immunoreactive to galanin was found in the adrenal tissue. A combination of HPLC analysis and RIA detection was used to characterize galanin-like immunoreactivity in frog adrenal gland extracts. The elution profile revealed the existence of a single form of galanin exhibiting the same retention time as synthetic frog galanin. The possible involvement of galanin in the regulation of corticosteroid secretion was investigated in vitro using a perifusion system for frog adrenal slices. For concentrations ranging from 10(-9) to 3 x 10(-6) M, synthetic frog galanin induced a dose-dependent inhibition of corticosterone and aldosterone release. Repeated pulses of galanin (10(-6) M), given at 90-min intervals, resulted in a reproducible inhibition of corticosteroid secretion without any apparent tachyphylaxis. During prolonged administration of galanin (10(-6) M), the steroidogenic effect of ACTH (10(-9) M) was significantly reduced. In contrast, galanin did not attenuate the stimulation of corticosteroid secretion induced by the angiotensin II a...Continue Reading

Citations

Apr 23, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Daniel M KeenanJohannes D Veldhuis
May 7, 1998·Endocrine Reviews·M Ehrhart-BornsteinG P Vinson
Dec 13, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·John Yuh-Lin YuYung-Hsi Kao

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