PMID: 7542757Apr 21, 1995Paper

Localization of 12-lipoxygenase mRNA in cultured oligodendrocytes and astrocytes by in situ reverse transcriptase and polymerase chain reaction

Neuroscience Letters
M K BendaniJ M Vallat

Abstract

We studied the distribution of 12-lipoxygenase mRNA in glial cells. First, mRNA was detected from cellular extracts by soluble-phase reverse transcriptase-polymerase chain reaction (RT-PCR). Taking into account that cell culture populations could not be 100% homogeneous, we then developed, for the first time, an in situ RT-PCR combined with immunocytochemistry with cell specific markers. Using this procedure we showed that 12-lipoxygenase mRNA was expressed both in mature oligodendrocytes and astrocytes.

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Citations

May 19, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·K A YoudimJ A Joseph
Dec 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ulrike HaasJesper Z Haeggström
Aug 3, 2010·Antioxidants & Redox Signaling·Cameron Rink, Savita Khanna
Sep 18, 2010·Journal of the American College of Nutrition·Chandan K SenSavita Khanna
Feb 6, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ann M GregusEdward A Dennis
Jan 28, 1998·Stroke; a Journal of Cerebral Circulation·D R HarderR J Roman
Oct 21, 2006·Stroke; a Journal of Cerebral Circulation·Klaus van LeyenEng H Lo
Jun 3, 2005·Journal of Neuroscience Research·Kyoung Ja KwonEun Joo Baik

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