PMID: 7029538Sep 1, 1981Paper

Localization of 3' ends of 5S and 23S rRNAs in reconstituted subunits of Escherichia coli ribosomes

Proceedings of the National Academy of Sciences of the United States of America
M Stöffler-MeilickeB Hardesty

Abstract

Periodate-oxidized 3' ends of 5S, 23S, and 16S rRNAs from Escherichia coli were allowed to react with fluorescein thiosemicarbazide, then labeled rRNAs were reconstituted into active ribosomal subunits. The fluorescein moiety on each of the rRNAs when reconstituted into ribosomal subunits was accessible to anti-fluorescein IgG as determined by fluorescence quenching and by sucrose gradient centrifugation. The region at which an antibody molecule bond to the labeled ribosomal subunits was determined by immunoelectron microscopy. The 3' end of the 5S RNA was localized on the central protuberance of the 50S subunit. The corresponding region for the 3' end of the 23S RNA was below the stalk on the noninterfacing surface. The 3' end of the 16S RNA was localized to the upper edge of the large lobe of 30S subunits, as reported previously.

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M Keren-ZurJ Ofengand
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H M Olson, D G Glitz
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G W TischendorfG Stöffler
Oct 15, 1975·FEBS Letters·A P CzernilofskyG Stöffler
Feb 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·M Sela, E Mozes
Dec 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·M Lubin
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·G Stöffler, H G Wittmann

❮ Previous
Next ❯

Citations

Apr 1, 1989·Molecular & General Genetics : MGG·M LottiG Stöffler
Jan 1, 1982·Progress in Biophysics and Molecular Biology·A Liljas
Jan 1, 1986·Progress in Biophysics and Molecular Biology·K Nagano, M Harel
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T C KingD Schlessinger
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M I OakesJ A Lake
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·O DontsovaR Brimacombe
Sep 24, 1983·Nucleic Acids Research·R W Richardson, R I Gumport
Feb 17, 2009·Biochemistry. Biokhimii︠a︡·G M GongadzeM B Garber
Feb 22, 2012·Biochemistry. Biokhimii︠a︡·G M Gongadze
Aug 21, 2008·Biological Chemistry·Romualdas StapulionisCharlotte R Knudsen
Jan 1, 1987·Journal of Molecular Evolution·G HarauzM van Heel
Dec 5, 1985·Journal of Molecular Biology·R Sirdeshmukh, D Schlessinger
Sep 22, 1982·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·H G Wittmann
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·A A BogdanovI N Lavrik

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.