PMID: 9423206Jan 10, 1998Paper

Localization of a bacterial infection with 99Tcm-labelled human IgG: further improvement with enriched IgG subclass preparations

Nuclear Medicine Communications
M WellingE K Pauwels

Abstract

The aim of this study was to determine the contribution of various IgG subclasses to the scintigraphic detection of a staphylococcal infection. An experimental thigh infection in mice was used to determine the accumulation of the various 99Tcm-labelled IgG preparations with enriched IgG1, IgG2 or IgG4 subclass. Multiple-regression analysis was used to investigate a relationship between the IgG subclasses and the time-dependent accumulation in infected sites. Eighteen hours after infection with Staphylococcus aureus bacteria 20 micrograms of 99Tcm-labelled IgG preparations enriched with one of the IgG1, IgG2 or IgG4 subclasses by thiophilic absorption were administered intravenously and target-to-nontarget (T/NT) ratios were determined at 15 min, 1 h, 4 h and 24 h after injection of the tracer. Moreover, the binding of these preparations to S. aureus was assessed using an in vitro bacterial pellet model as an indication for the potency of detecting infections. As a control agent, 99Tcm-labelled polyclonal IgG (HIG) was used. In vivo, the T/NT ratios were significantly (P < 0.05) higher for the IgG1-enriched preparation at all time points, and for the IgG2-enriched preparation at 4 h and 24 h after injection, compared with HIG. I...Continue Reading

Citations

Oct 18, 2012·Metallomics : Integrated Biometal Science·Dipak Kumar NayakMita Chatterjee Debnath
Jun 26, 2015·FEMS Microbiology Reviews·Marleen van OostenGooitzen M van Dam

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