Localization of a K+ -binding site involved in dephosphorylation of the sarcoplasmic reticulum Ca2+ -ATPase

The Journal of Biological Chemistry
Thomas Lykke-Møller SørensenPoul Nissen

Abstract

K+ plays an important role for the function of the sarco(endo)plasmic reticulum Ca2+ -ATPase (SERCA), but its binding site within the molecule has remained unidentified. We have located the binding site for a K+ ion in the P-domain by means of x-ray crystallography using crystals prepared in the presence of the K+ congener Rb+. Backbone carbonyls from the loop containing residues 711-715 together with the side chain of Glu732 define the K+/Rb+ site in the Ca2+ -ATPase conformation with bound Ca2+, ADP, and AlF4-. Functional analysis of Ca2+ -ATPase mutants with alterations to Glu732 shows that this site is indeed important for the stimulatory effect of K+ on the dephosphorylation rate. Comparison with the Ca2+ -ATPase in a dephosphorylated E2 conformation suggests that the K+ site is involved in the correct movement and positioning of the A-domain during translocation and dephosphorylation.

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Citations

Sep 3, 2010·Quarterly Reviews of Biophysics·Jesper V MøllerPoul Nissen
Dec 24, 2010·Nature Reviews. Molecular Cell Biology·J Preben MorthPoul Nissen
Oct 13, 2010·The Journal of Biological Chemistry·Kazuo YamasakiHiroshi Suzuki
Mar 1, 2011·Annual Review of Biophysics·Michael G Palmgren, Poul Nissen
Oct 24, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Amanda N BarrySvetlana Lutsenko
May 13, 2006·Journal of Bioenergetics and Biomembranes·Giuseppe InesiCarlota Sumbilla
Apr 10, 2012·Molecular and Cellular Probes·Maria-Barbara LeporiGeorgios Loudianos
Jan 21, 2012·Chemical Biology & Drug Design·Mukesh Chourasia, G Narahari Sastry
Aug 12, 2014·The FEBS Journal·Nikolaj D DrachmannMaike Bublitz
Jun 19, 2008·Archives of Biochemistry and Biophysics·Larry D Faller
Apr 7, 2016·Structure·Johannes D ClausenPoul Nissen
Feb 22, 2012·Biophysical Journal·Maria MusgaardEmad Tajkhorshid
Jul 13, 2005·Current Opinion in Structural Biology·Jesper V MøllerMarc le Maire
Jan 25, 2018·The Journal of Biological Chemistry·Stine A MikkelsenJens Peter Andersen
Jun 24, 2018·The Journal of Biological Chemistry·Eli Fernández-de Gortari, L Michel Espinoza-Fonseca
May 13, 2006·Journal of Bioenergetics and Biomembranes·Jesper Vuust MøllerPoul Nissen
Feb 21, 2019·Molecular Biology of the Cell·Danny Mollerup SørensenMichael Palmgren
Aug 2, 2008·The Journal of Biological Chemistry·Vivien Rodacker SchackBente Vilsen
Aug 9, 2006·The Journal of Biological Chemistry·Anne Nyholm AnthonisenJens Peter Andersen
Jun 6, 2013·The Journal of Biological Chemistry·Kazuo YamasakiHiroshi Suzuki
Jun 10, 2008·The Journal of Biological Chemistry·Yasser A Mahmmoud
Jun 14, 2020·International Journal of Molecular Sciences·Rodrigo Aguayo-Ortiz, L Michel Espinoza-Fonseca
Oct 24, 2006·The Journal of Biological Chemistry·Morten J Buch-PedersenMichael G Palmgren
May 24, 2021·Journal of Molecular Biology·Milena TimcenkoPoul Nissen
Feb 27, 2019·Journal of Chemical Theory and Computation·Bin SunPeter M Kekenes-Huskey
Mar 11, 2008·Archives of Biochemistry and Biophysics·Francesco Tadini-BuoninsegniKlaus Fendler
Aug 27, 2009·Archives of Biochemistry and Biophysics·Sanja BecaGary J Kargacin
Dec 25, 2004·Science·Claus OlesenPoul Nissen

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