Abstract
Metal binding sites within the Gla domain of vitamin K-dependent coagulation factors have been divided into nonspecific metal sites and calcium-specific sites. We demonstrate here that five residues within the Gla domain of factor IX are responsible for the reactivity with the metal-dependent factor IX monoclonal antibody, A-7. First we demonstrate that modifying any one of three residues within this site in factor IX abolishes the binding of A-7. To confirm the specificity of the antibody, the Gla domain of factor VII was changed at residues 32, 33, 34, 38 and 39 to the homologous residues of human factor IX. These changes were sufficient to generate a factor VII Gla domain with an A-7 binding site of the same affinity as that in factor IX. The site identified is one of the two major surfaces of the Gla domain and may represent the metal-dependent binding site.
References
Jan 1, 1977·Annual Review of Biochemistry·J Stenflo, J W Suttie
Oct 17, 1978·Biochemistry·J W Bloom, K G Mann
Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Mar 10, 1992·Biochemistry·M Soriano-GarciaA Tulinsky
Jun 15, 1990·Thrombosis Research·T SugoM Matsuda
Jun 26, 1989·Nucleic Acids Research·P StanssensH J Fritz
Mar 1, 1987·American Journal of Clinical Pathology·K J SmithL F Smith
Jan 1, 1985·Annual Review of Biochemistry·J W Suttie
Jan 1, 1985·Haematologia·C Vermeer, M A De Boer-Van den Berg
Jan 15, 1984·Thrombosis Research·K J Smith, K Ono
Feb 15, 1983·Biochemistry·R M LewisB Furie
Jun 1, 1995·Nature Structural Biology·M SunnerhagenJ Stenflo
Mar 1, 1993·European Journal of Biochemistry·H A Liebman
Jun 16, 1995·The Journal of Biological Chemistry·F SekiyaT Morita
Oct 11, 1994·Biochemistry·S S AhmadP N Walsh
Feb 8, 1994·Biochemistry·T P SeshadriA Tulinsky
Citations
Jan 1, 1997·Protein Science : a Publication of the Protein Society·S GillisM D Bond
Jan 1, 1996·Thrombosis Research·W F CheungT Sugo
Mar 21, 1998·Biochimica Et Biophysica Acta·E G WojcikR M Bertina
Aug 22, 2008·Blood·Junjiang SunPaul E Monahan
Apr 26, 2000·The Journal of Biological Chemistry·M A BrownJ Stenflo
Nov 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Anders L NielsenHenrik Østergaard
Aug 23, 2003·Blood·Karen E RussellTimothy C Nichols
Feb 19, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Chi Ming Yang
Aug 9, 2020·Blood Advances·Wenwen GaoGuomin Shen