Abstract
Four cDNA fragments encoding different portions of the alpha-subunit of human H,K-adenosine triphosphatase (ATPase) were amplified by means of the polymerase chain reaction technique, ligated into the plasmid pGEX-2T, and expressed as glutathione S-transferase fusion proteins in Escherichia coli. The fragments A (residues 163-313), Ba (residues 360-797), Bb (residues 526-797), and C (residues 822-1031) together encompass 77% of the alpha-subunit and cover most of its cytosolic part. The reactivities of autoantibodies in the sera from patients with pernicious anaemia with the recombinant fusion proteins were analysed by immunoblotting. One autoantigenic epitope was found in the NH2-terminal part of the Ba fragment--that is, between residues 360 and 525. No epitope was detected in the other fragments. The Ba fragment was cleaved off from the glutathione S-transferase fusion protein by the action of thrombin and was then further purified. By means of enzyme-linked immunosorbent assay, 28 of 42 sera (67%) from patients with pernicious anaemia were positive against the purified Ba fragment. The present results provide a final proof that the human H,K-ATPase alpha-subunit is a major autoantigen in the parietal cell and that the major...Continue Reading
References
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Aug 1, 1991·Gastroenterology·C M JonesB H Toh
Jul 1, 1991·Immunology Today·P A Gleeson, B H Toh
Oct 15, 1991·Biochemical and Biophysical Research Communications·J Y MaS Mårdh
Oct 1, 1991·Scandinavian Journal of Gastroenterology·S MårdhK Henriksson
Mar 1, 1990·Biochimica Et Biophysica Acta·C T OkamotoJ G Forte
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B H TohD C Humphris
Jan 1, 1990·Annual Review of Physiology·E C Rabon, M A Reuben
Jan 23, 1990·Biochemistry·K HallG Sachs
Aug 1, 1989·Acta Physiologica Scandinavica·S Mårdh, Y H Song
Jun 1, 1989·Gastroenterology·P BurmanF A Karlsson
Feb 1, 1988·The Journal of Clinical Investigation·F A KarlssonS Mårdh
Dec 1, 1987·Acta Physiologica Scandinavica·S MårdhT Björkman
Jul 15, 1988·Gene·D B Smith, K S Johnson
Dec 1, 1965·The Journal of Clinical Investigation·G H Jeffries, M H Sleisenger
May 1, 1973·The American Journal of Digestive Diseases·R G Strickland, I R Mackay
Nov 1, 1983·Gene·U Gubler, B J Hoffman
Sep 1, 1983·Clinical Immunology and Immunopathology·M D KayeR Wright
Jan 11, 1984·Biochimica Et Biophysica Acta·M LjungströmS Mårdh
Nov 24, 1962·British Medical Journal·K B TAYLORC SHAPLAND
Citations
May 1, 1996·Immunology Today·Y H SongN K Maclaren
May 2, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Erik MårdhKurt Borch
Aug 17, 2002·Alimentary Pharmacology & Therapeutics·M ItoK Chayama
Feb 20, 2002·Journal of Gastroenterology and Hepatology·K Dohmen
May 1, 2002·European Journal of Gastroenterology & Hepatology·Masanori ItoKazuaki Chayama
Apr 30, 2014·Clinical and Experimental Immunology·A ChobotM Rewers
Jan 20, 2017·Clinical and Translational Gastroenterology·Edith LahnerVito Lampasona
Sep 24, 2005·Digestion·Yuji NaitoHidekazu Suzuki
Sep 1, 1994·Scandinavian Journal of Gastroenterology·J Y MaS Mårdh
Jul 11, 2020·Nature Reviews. Disease Primers·Marco Vincenzo LentiAntonio Di Sabatino
Nov 1, 1994·Scandinavian Journal of Gastroenterology·J Y MaS Mårdh