PMID: 6974569Jul 27, 1981Paper

Localization of A protein in the RNA-A protein complex of RNA phage MS2

Biochimica Et Biophysica Acta
T Shiba, Y Suzuki

Abstract

The site of interaction of phage MS2 A protein on MS2 RNA was determined by analysing the base sequence of the RNA fragment which was released from the RNAase-resistant complex prepared by RNAase digestion of RNA-A protein complex. The result showed that there were two types of RNA fragment: one had a sequence which corresponded to the sequence of the 5'-side maturation region of MS2 RNA and the other corresponded to the sequence of the 3'-side untranslated region. These results were confirmed by a competition experiment in in vitro reconstitution system using f2 defective RNA, lacking about 30% of the 5'-side, and MS2 5FUra (5-fluorouracil) RNA, lacking about 35% of the 3'-side, as competitors. These results seem to indicate that the A protein is bound to at least two sites on MS2 RNA.

References

Jul 24, 1975·Nature·W FiersM Van Montagu
Mar 13, 1975·Nature·T Shiba, T Miyake
Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·A Bernardi, P F Spahr
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·T MiyakeK Yamane

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Citations

May 2, 2013·Plasmid·Denis Arutyunov, Laura S Frost
Sep 25, 1993·Nucleic Acids Research·G G Pickett, D S Peabody
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Jan 1, 1986·Progress in Biophysics and Molecular Biology·L Liljas
Apr 27, 2017·The Science of the Total Environment·Wakana OishiNaoyuki Funamizu
Aug 26, 2020·Progress in Biophysics and Molecular Biology·Karl Victor Gorzelnik, Junjie Zhang
Aug 27, 1990·Biochimica Et Biophysica Acta·M R AdhinJ van Duin
Feb 17, 2011·Environmental Science & Technology·Brian M PecsonTamar Kohn

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