PMID: 8980293Jan 1, 1997Paper

Localization of aminopeptidase activity in freshly excised human skin: direct visualization by confocal laser scanning microscopy

The Journal of Investigative Dermatology
P BoderkeH E Boddé

Abstract

The aim of this study was to localize and visualize aminopeptidase activity within freshly excised, dermatomed human skin without perturbation of its histologic integrity. The use of confocal laser scanning microscopy (CLSM) is introduced as a novel approach by which to monitor the degradation of suitable substrates in the skin. The fluorescence of the metabolites originating from the cleavage of the aminopeptidase probe bis-Leu-rhodamine 110 (Leu2-R11O) was interpreted to reflect the local aminopeptidase activity in the tissue. To separate the kinetics of diffusion and degradation of Leu2-R110, a lateral application mode was introduced: the probe was applied at the cutting plane of a mechanical cross-section of the sample, and optical cross-sections were made parallel to the cutting plane of the mechanical section. By this means, simultaneous and equal access of the substrate to the various strata and domains of the skin was achieved. The observations revealed that the fluorescence, i.e., aminopeptidase activity, was evenly distributed throughout the viable part of the epidermis, with enhanced fluorescence ("hot spots") in the upper layers of the stratum granulosum, while dermis and stratum corneum showed considerably less ami...Continue Reading

Citations

May 4, 2011·International Journal of Cosmetic Science·N H Abu Samah, C M Heard
Jul 20, 2006·Pharmaceutical Research·Nada AblaYogeshvar N Kalia
Dec 28, 2006·Skin Pharmacology and Physiology·E HoubenV Rogiers
Sep 20, 2005·Pharmaceutical Research·Nada AblaYogeshvar N Kalia
Sep 25, 1999·International Journal of Cancer. Journal International Du Cancer·L H BrinckerhoffC L Slingluff
Sep 13, 2006·Archives of Dermatological Research·Ute JacobiJürgen Lademann
Nov 1, 2007·The British Journal of Dermatology·M BlountP Slusarewicz
Feb 18, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·D Imbert, C Cullander
Jun 6, 2000·Advanced Drug Delivery Reviews·H P MerkleM C Schmidt
Aug 7, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·R Alvarez-RománR H Guy

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