PMID: 6159643Sep 1, 1980Paper

Localization of androgen-binding protein in proliferating Sertoli cells in culture

Proceedings of the National Academy of Sciences of the United States of America
A L KierszenbaumF S French

Abstract

The peroxidase and immunofluorescent localization patterns of androgen-binding protein (ABP), a biological marker of Sertoli cell function, have been examined in cultured Sertoli cells isolated from 20- to 22-day-old rats. ABP immunoreactivity in the form of cytoplasmic granules of variable diameter was observed in Sertoli cells with characteristic lipid droplets and a colony-forming, epithelial-like growth pattern. Incubation of cultures with [3H]thymidine demonstrated that Sertoli cells continue to produce ABP while retaining their capability for synthesizing DNA and undergoing mitosis. A variable number of cultured Sertoli cells became morphologically transformed after exposure to follitropin (follicle-stimulating hormone) and pharmacological agents acting on cyclic nucleotide metabolism. The induced change in Sertoli cell shape coincided with a disappearance of ABP-containing granules from the cytoplasm. These observations demonstrate that localization of ABP by immunological techniques is a valuable tool for the characterization of structural and functional properties of Sertoli cell in culture.

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Citations

Jan 1, 1982·Molecular and Cellular Endocrinology·E M RitzenM Feldman
Feb 18, 1999·Molecular and Cellular Endocrinology·S ScarpinoC Boitani
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A L KierszenbaumL L Tres
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R M DePhilip, A L Kierszenbaum
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L L Tres, A L Kierszenbaum
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·W A SpruillA L Kierszenbaum
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N T LeeA L Kierszenbaum
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D R JosephF S French
Jan 1, 1987·Annals of the New York Academy of Sciences·A L Kierszenbaum, L L Tres
Jun 1, 1986·Journal of Cellular Physiology·M AbdullahA L Kierszenbaum
Jan 1, 1986·Developmental Genetics·D K LeBarr, S R Blecher
May 5, 2021·Expert Review of Molecular Diagnostics·Xu YouHaixia Zhao

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