PMID: 9179436May 1, 1997Paper

Localization of antigenic sites on Der p 2 using oligonucleotide-directed mutagenesis targeted to predicted surface residues

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
A M Smith, M D Chapman

Abstract

Understanding the molecular nature of allergen-antibody interactions is important to understanding the mechanism of conventional immunotherapy as well as to designing alternative immunotherapeutic strategies. Many important allergens have been cloned and expressed, making it possible to apply recombinant DNA techniques to dissect antigenic determinants. The aim of this study was to use predictive algorithms and site-directed mutagenesis to investigate monoclonal antibody and IgE antibody epitopes of the major house dust mite allergen Der p 2. Computer algorithms were used to assess the primary amino acid sequence of Der p 2 and to identify regions of hydrophilic and flexible sequence. Subsequently, site-directed mutagenesis was used to generate amino acid substitutions at hydrophilic residues at positions 44-46 and at position 100. The variants were tested in a competitive inhibition ELISA with four group 2-specific murine monoclonal antibodies and with human IgE antibody from mite allergic patients. Conservative amino acid substitutions at position 44-46 did not distinguish IgE antibody epitopes, but did suggest that these residues are involved in the epitope defined by one monoclonal antibody, 15E11. Non-conservative substitu...Continue Reading

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Citations

Apr 27, 2012·Experimental & Applied Acarology·Kyoung Yong JeongJung-Won Park
Apr 22, 2005·Current Allergy and Asthma Reports·Wayne R ThomasWendy-Anne Smith
May 10, 2003·Journal of Immunological Methods·Wei-Jen TsaiChiou-Ying Yang
Nov 4, 2000·Biochimica Et Biophysica Acta·R MäntyjärviT Virtanen
Nov 24, 2007·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·J BousquetP Demoly
Jul 27, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S Fearby, A J Frew
Jul 9, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J W ParkC-S Hong
Sep 27, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jill GlesnerAnna Pomés
Feb 24, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F FerreiraO Scheiner
Oct 10, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B J HalesW R Thomas
Nov 28, 2018·Allergy, Asthma & Immunology Research·Bhakkawarat KulwanichSurapon Piboonpocanun
Aug 24, 2019·Scientific Reports·Kavita ReginaldFook Tim Chew
Sep 11, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Geoffrey A MuellerAnna Pomés

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