PMID: 8609375Feb 1, 1996Paper

Localization of apoptotic cells by direct immunogold detection of digoxigenin-labeled genomic DNA in semithin sections

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
L Van NassauwM Callebaut

Abstract

In this study, which correlates apoptosis with avian ovarian physiology, we modified an in situ DNA nick end-labeling method using immunogold reagents to detect apoptotic cells in semithin sections of quail ovaries embedded in glycol methacrylate resin. Special attention was paid to the prevention of background staining. The results are comparable with those of the ApopTag peroxidase kit in paraffin-embedded ovaries.

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis