Localization of ATP-sensitive K+ channel subunits in rat submandibular gland.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Ming ZhouHiroshi Abe

Abstract

ATP-sensitive K(+) (K(ATP)) channel subunits were investigated in rat submandibular gland (SMG). RT-PCR detected the presence of mRNA transcripts of the Kir6.1, Kir6.2, SUR2A, and SUR2B in the SMG, whereas SUR1 mRNA was barely detected. Western blot analysis provided the evidence that these four K(ATP) channel subunits are expressed in rat SMG. Immunostaining detected that these four K(ATP) channel subunits are widely distributed, with different intensities, in myoepithelial cells, epithelial cells of intercalated ducts, granular convoluted tubules, striated ducts, and excretory ducts. Immunofluorescence double staining showed that Kir6.1 and Kir6.2 colocalized with SUR2A in the myoepithelial cells, granular convoluted tubules, striated ducts, and excretory ducts. Kir6.1 and Kir6.2 also colocalized with SUR2B, mainly in the duct system, e.g., the granular convoluted tubules, striated ducts, and excretory ducts. Taken together, these results indicate that the K(ATP) channels in SMG may consist of Kir6.1, Kir6.2, SUR2A, and SUR2B, with various combinations of colocalization with each other, and may play important roles in rat SMG during salivary secretion.

References

Sep 1, 1992·Neurochemistry International·T YamamotoT Amano
Dec 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·O AmanoS Iseki
Aug 1, 1988·Pflügers Archiv : European journal of physiology·M L AshfordC N Hales
Aug 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T Barka
Jan 1, 1994·Microscopy Research and Technique·E W Gresik
Sep 1, 1993·Cell and Tissue Research·O AmanoS Iseki
Oct 4, 1996·The Journal of Biological Chemistry·S IsomotoY Kurachi
Feb 1, 1997·Endocrinology·C Hernández-SánchezD LeRoith
May 1, 1997·Neuron·J P ClementJ Bryan
Oct 23, 1997·The Journal of Physiology·G ChaturapanichD I Cook
Sep 23, 1998·Brain Research Bulletin·B E Levin, A A Dunn-Meynell
Apr 16, 1999·Endocrine Reviews·L Aguilar-Bryan, J Bryan
Aug 3, 1999·Journal of Cellular Physiology·N ChaïbJ P Dehaye
Jun 2, 2000·The Journal of Physiology·M Mederos y SchnitzlerR Preisig-Müller
Feb 5, 2003·Progress in Biophysics and Molecular Biology·Susumu Seino, Takashi Miki
Mar 27, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Laurianne van BeverAlex J Baertschi
Jun 29, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ming ZhouHiroshi Abe
Feb 22, 2007·American Journal of Physiology. Endocrinology and Metabolism·Maricedes Acosta-Martínez, Jon E Levine
Apr 18, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ming ZhouHiroshi Abe
Jan 25, 2008·American Journal of Physiology. Cell Physiology·Tetsuji NakamotoJames E Melvin
Mar 8, 2008·The Tohoku Journal of Experimental Medicine·Ming ZhouHiroshi Abe

❮ Previous
Next ❯

Citations

Dec 25, 2012·Pflügers Archiv : European journal of physiology·Ervice PouokamMartin Diener

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.