Localization of beta-phycoerythrin to the thylakoid lumen of Cryptomonas phi does not involve a signal peptide

Plant Molecular Biology
M Reith, S E Douglas

Abstract

Recent investigations have shown that, in cryptomonads, the phycobiliproteins are located within the thylakoid lumen rather than on the stromal side of this membrane as found in cyanobacteria and red algae. To examine possible mechanisms involved in targeting this protein to the thylakoid lumen, the plastid-encoded cpeB gene from Cryptomonas phi was sequenced. This gene encodes an open reading frame of 177 amino acids that is highly similar to known beta-phycoerythrin proteins. cpeB is expressed as a monocistronic transcript of approximately 680 bases. The genes for the alpha subunits of phycoerythrin are not co-transcribed with cpeB nor located anywhere near it. No evidence of amino- or carboxy-terminal extensions or interior modifications involved in directing the Cryptomonas beta-phycoerythrin into the lumen of the thylakoid could be detected. These data suggest that a novel mechanism may be involved in directing cryptomonad biliproteins to the thylakoid lumen.

References

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Oct 27, 1975·Archives of Microbiology·E Mörschel, W Wehrmeyer
Jun 11, 1990·Nucleic Acids Research·C J Howe, T P Wallace
Nov 15, 1986·Analytical Biochemistry·L I Siegel, E Bresnick
Sep 9, 1988·Science·K Verner, G Schatz
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D EisenbergT C Terwilliger
Jan 1, 1982·Annual Review of Microbiology·A N Glazer
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jun 10, 1980·Biochimica Et Biophysica Acta·C LichtléJ C Duval
Jan 1, 1986·Plant Physiology·D Guard-FriarR Maccoll

❮ Previous
Next ❯

Citations

Nov 1, 1995·Photosynthesis Research·A N Glazer, G J Wedemayer
Sep 1, 1991·Journal of Molecular Evolution·S E Douglas, S Turner
Mar 27, 1992·Biochimica Et Biophysica Acta·J Godovac-ZimmermannR G Hiller
Nov 1, 1992·Trends in Ecology & Evolution·C J HoweP J Lockhart
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C BernardJ P Dubacq
Jan 16, 1999·The Journal of Biological Chemistry·J C Thomas, C Passaquet
Mar 14, 1991·Nature·D Penny, C J O'Kelly
Aug 19, 2007·The Journal of Biological Chemistry·Sven B GouldRalf Bernd Klösgen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.