Localization of betav tubulin in the cochlea and cultured cells with a novel monoclonal antibody

Cell Motility and the Cytoskeleton
Asok BanerjeeR F Ludueña

Abstract

Tubulin, the dimeric structural protein of microtubules, is a heterodimer of alpha and beta subunits; both alpha and beta exist as numerous isotypes encoded by different genes. In vertebrates the sequence differences among the beta(I), beta(II), beta(III), beta(IV) and beta(V) isotypes are highly conserved in evolution, implying that the isotypes may have functional significance. Isotype-specific monoclonal antibodies have been useful in determining the cellular and sub-cellular distributions and possible functions of the beta(I), beta(II), beta(III), and beta(IV) isotypes; however, little is known about the beta(V) isotype. We here report the creation and purification of a monoclonal antibody (SHM.12G11) specific for beta(V). The antibody was designed to be specific for the C-terminal sequence EEEINE, which is unique to rodent and chicken beta(V). The antibody was found to bind specifically to the C-terminal peptide EEEINE, and does not cross-react with the carboxy-termini of either alpha-tubulin or the other beta-tubulin isotypes. However, the antibody also binds to the peptide EEEVNE, but not to the peptide EEEIDG, corresponding respectively to the C-terminal peptides of bovine and human beta(V). Immunofluorescence analysis ...Continue Reading

References

Aug 15, 1977·European Journal of Biochemistry·A FellousJ Nunez
May 1, 1990·Molecular and Cellular Biology·K M PollardC A Glass
Jan 1, 1988·Annual Review of Cell Biology·K F Sullivan
Feb 5, 1988·Journal of Molecular Biology·M J Monteiro, D W Cleveland
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K F Sullivan, D W Cleveland
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·I Gozes, C J Barnstable
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·D PandaL Wilson
Dec 31, 1997·International Review of Cytology·R F Ludueña
May 8, 1998·Biochemical and Biophysical Research Communications·A Banerjee, L T Kasmala
Jan 27, 1999·Biochemical and Biophysical Research Communications·A BanerjeeK H Lee
Sep 9, 2000·Hearing Research·R Hallworth, R F Ludueña
Dec 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert L StrausbergUNKNOWN Mammalian Gene Collection Program Team
Feb 15, 2003·Blood·Joseph E ItalianoRamesh A Shivdasani
May 13, 2003·Cell Motility and the Cytoskeleton·Christos D KatsetosSverre J Mörk
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·J Hartwig, J Italiano
Oct 25, 2003·Oncogene·George A OrrSusan Band Horwitz
Jan 7, 2004·Journal of Neurocytology·Heather C Jensen-SmithRichard Hallworth
Sep 15, 2005·Journal of Cell Science·Julia VentRichard Hallworth
Nov 30, 2005·Biochemistry·Pascal Verdier-PinardSusan Band Horwitz

❮ Previous
Next ❯

Citations

Jul 27, 2015·Histochemistry and Cell Biology·Justine RenauldMarc Thiry
Oct 9, 2008·Analytical Biochemistry·Pascal Verdier-PinardDiane Braguer
Jan 12, 2017·Cytotechnology·Ana Carolina M SantosMaria Angélica Miglino

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.