Localization of BRCA1 and a splice variant identifies the nuclear localization signal.

Molecular and Cellular Biology
S ThakurB L Weber

Abstract

Inherited mutations in BRCA1 confer susceptibility to breast and ovarian neoplasms. However, the function of BRCA1 and the role of BRCA1 in noninherited cancer remain unknown. Characterization of alternately spliced forms of BRCA1 may identify functional regions; thus, we constructed expression vectors of BRCA1 and a splice variant lacking exon 11, designated BRCA1 delta 672-4095. Immunofluorescence studies indicate nuclear localization of BRCA1 but cytoplasmic localization of BRCA1 delta 672-4095. Two putative nuclear localization signals (designated NLS1 and NLS2) were identified in exon 11; immunofluorescence studies indicate that only NLS1 is required for nuclear localization. RNA analysis indicates the expression of multiple, tissue-specific forms of BRCA1 RNAs; protein analysis with multiple antibodies suggests that at least three BRCA1 isoforms are expressed, including those lacking exon 11. The results suggest that BRCA1 is a nuclear protein and raise the possibility that splicing is one form of regulation of BRCA1 function by alteration of the subcellular localization of expressed proteins.

References

Oct 1, 1989·Molecular and Cellular Biology·M Hannink, H M Temin
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Oct 7, 1994·Science·P A FutrealY Miki
Jun 11, 1993·Annals of the New York Academy of Sciences·P S Freemont
Mar 1, 1996·Nature Genetics·J T HoltR A Jensen
Mar 1, 1996·Nature Genetics·R A JensenJ T Holt
Apr 5, 1996·Science·R ScullyD M Livingston
Dec 1, 1995·Human Molecular Genetics·S K SharanA Bradley

❮ Previous
Next ❯

Citations

Jun 19, 2001·Cancer Investigation·E M RosenI D Goldberg
Dec 27, 2012·Cancer Letters·Anders Christiansen, Lars Dyrskjøt
Dec 4, 2012·Experimental Cell Research·Kevin W Caestecker, Gerlinde R Van de Walle
Apr 13, 2007·Experimental Cell Research·Cimona V HintonMarilyn E Thompson
Jun 19, 2008·International Journal of Cancer. Journal International Du Cancer·Oleg GluzArndt Hartmann
Jul 9, 2010·The FEBS Journal·Marilyn E Thompson
Jul 30, 2005·Cancer Letters·Blase Billack, Alvaro N A Monteiro
Jul 6, 2004·Clinical Biochemistry·Brigitta M N Brinkman
Jul 15, 2004·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·N ChalabiD J Bernard-Gallon
Jul 22, 2004·Experimental Cell Research·Megan FabbroBeric R Henderson
Dec 13, 2005·Critical Reviews in Oncology/hematology·Marzia PalmaAlberto Gulino
Sep 28, 2005·Biochimica Et Biophysica Acta·Jessyka FortinJacques Simard
Sep 1, 2000·Seminars in Cancer Biology·B ArverA Lindblom
Apr 9, 2014·Biochimica Et Biophysica Acta·Xiaohong Wang, Shulin Li
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·B H SpainI M Verma
Jul 2, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Johanne I WeberpalsBarbara C Vanderhyden
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·H Ruffner, I M Verma
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y JinR Baer
Jan 20, 2004·Experimental Cell Research·José Antonio RodriguezBeric R Henderson
Jan 6, 2009·Biochemistry. Biokhimii︠a︡·Lixia MiaoCongyi Zheng
Feb 26, 2014·Journal of Lipid Research·Kathryn C JacksonEspen E Spangenburg
May 6, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Fahd Al-MullaJehoram T Anim
Nov 4, 2000·International Journal of Cancer. Journal International Du Cancer·R BachelierA Vincent
Aug 4, 1998·International Journal of Cancer. Journal International Du Cancer·D J Bernard-GallonY J Bignon
Jul 27, 1999·International Journal of Cancer. Journal International Du Cancer·D J Bernard-GallonY J Bignon
Oct 6, 2017·Genes·Nancy Martínez-MontielRebeca D Martínez-Contreras
Apr 26, 2013·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Mohamed HassaneinHagay Sobol
Jul 8, 2000·The Journal of Biological Chemistry·T K MacLachlanW S El-Deiry
Jul 9, 2002·Oncogene·Marion KubistaMarkus Hengstschläger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Cancer: BRCA1 & BRCA2

Mutations involving BRCA1, found on chromosome 17, and BRCA2, found on chromosome 13, increase the risk for specific cancers, such as breast cancer. Discover the last research on breast cancer BRCA1 and BRCA2 here.