PMID: 7581260Aug 1, 1995Paper

Localization of calcium channels of the L-type in human epicardial arteries: a light microscope autoradiographic study

Clinical and Experimental Hypertension : CHE
F FerranteF Amenta

Abstract

The anatomical localization of Ca2+ channels of the L-type was analyzed in sections of the human right and anterior interventricular coronary arteries by using in vitro light microscope autoradiography associated with radioligand binding techniques. [3H]Nicardipine was utilised as a ligand. Binding of the radioligand to sections of the two coronary arteries was time-, temperature- and concentration-dependent. Analysis of binding isotherms revealed a dissociation constant value of about 0.5 nM in the two arteries and maximum binding capacities of 139 +/- 6.4 fmol/mg tissue for the right coronary artery and of 173 +/- 9.5 for the anterior interventricular branch. The pharmacological profile of [3H]nicardipine binding to sections of human coronary arteries was consistent with the labelling of Ca2+ channels of the L-type. Dihydropyridine derivatives were the most powerful competitors of [3H]nicardipine binding, whereas phenylalkylamines, benzothiazepine or non-selective channel modulators were weak competitors or ineffective. Light microscope autoradiography revealed the highest density of [3H]nicardipine binding sites in the tunica media of the coronary arteries. In this layer Ca2+ channels of the L-type are located within smooth ...Continue Reading

References

Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R B RogartV J Dzau
Nov 7, 1989·The American Journal of Cardiology·I GraziadeiH Glossmann
Jan 1, 1985·Methods in Enzymology·H Glossmann, D R Ferry
Jan 1, 1988·Trends in Pharmacological Sciences·T GodfraindM Wibo
Jan 22, 1982·European Journal of Pharmacology·K M Murphy, S H Snyder
Aug 24, 1993·European Journal of Pharmacology·F Ferrante, F Amenta
Feb 1, 1993·Naunyn-Schmiedeberg's Archives of Pharmacology·F AmentaA Ricci

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Citations

Jun 2, 1998·Clinical and Experimental Hypertension : CHE·F AmentaA Ricci
Mar 29, 2001·Clinical and Experimental Hypertension : CHE·F AmentaD Vitali

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