PMID: 6411806Sep 1, 1983Paper

Localization of calcium in red blood cells

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
M BorgersF De Clerck

Abstract

The distribution of calcium is demonstrated in human red blood cells (RBC) with a combined phosphate-pyroantimonate technique (PPA). Freshly collected blood and tissue biopsies were initially fixed in potassium phosphate-glutaraldehyde and the complexed calcium was subsequently visualized on Vibratome sections with potassium pyroantimonate. The majority of cells, both in isolated as well as "in situ" preparations, show a fine granular precipitate located at the inner leaflet of the plasma membrane. A minority of cells lack these membrane-associated deposits, exhibiting instead a random distribution of very fine precipitate in their cytoplasm. Capillary endothelial cells and pericytes are devoid of plasma membrane-bound precipitate. When irreversible crenation of RBC is induced by exposure to ionophore A 23187 and calcium, the sphero-echinocytes loose their membrane-bound precipitate, whereas the cells that retain their discocyte shape demonstrate the usual pattern of membrane-bound deposits. Contrarily, cells showing reversible shape changes induced by either A 23187-Ca2+ challenge, by adenosine triphosphate depletion during aging, or contact with lysolecithin, retain or regain the membrane-bound calcium. This cytochemical demo...Continue Reading

Citations

Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·S E JorgensenC F Louis
Aug 1, 1990·Journal of Clinical Pathology·H G OlbrichG Kober
Mar 1, 1984·The Histochemical Journal·M BorgersH E Ter Keurs
Sep 1, 1983·The American Journal of Emergency Medicine·M BorgersF Verheyen
Jan 1, 1986·Journal of Ultrastructure and Molecular Structure Research·R C HertsensW A Jacob

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