PMID: 6989840May 1, 1980Paper

Localization of calmodulin in rat cerebellum by immunoelectron microscopy

The Journal of Cell Biology
C T LinA R Means

Abstract

Calmodulin, a multifunctional Ca(++)-binding protein, is present in all eucaryotic cells. We have investigated the distribution of this protein in the rat cerebellum by immunoelectron microscopy using a Fab-peroxidase conjugate technique. In Purkinje and granular cell bodies, calmodulin reaction product was found localized both on free ribosomes and on those attached to rough endoplasmic reticulum (RER) and the nuclear envelope. No calmoduline was observed in the cisternae of RER or the Golgi apparactus. Calmodulin did not appear to be concentrated in the soluble fraction of the cell under the conditions used. Rather, peroxidase reaction product could be seen associated with membranes of the Golgi apparatus the smooth endoplasmic reticulum (SER), and the plasma membrane of both cell bodies and neuronal processes. In the neuronal dendrites, calmodulin appeared to be concentrated on membranes of the SER, small vesicles, and mitochondria. Also, granular calmodulin was observed in the amorphous material. In the synaptic junction, a large amount of calmodulin was seen attached to the inner surface of the postsynaptic membrane, whereas very little was observed in the presynaptic membrane or vesicles. These observations suggest that c...Continue Reading

References

Aug 1, 1975·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C T LinJ P Chen
Oct 31, 1975·Science·C Lin, J Chang
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M J WelshA R Means
Oct 29, 1979·Biochemical and Biophysical Research Communications·T Yamauchi, H Fujisawa
Dec 29, 1978·Biochemical and Biophysical Research Communications·R Dabrowska, D J Hartshorne
Jan 1, 1976·Cold Spring Harbor Symposia on Quantitative Biology·W R Loewenstein
Jan 1, 1970·Advances in Biochemical Psychopharmacology·W Y Cheung
Jan 1, 1972·Endocrinology·A R Means, J Vaitukaitis
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J F HarperA L Steiner

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Citations

Jan 1, 1983·Histochemistry·A Seto-OhshimaA Mizutani
Sep 1, 1991·Journal of Neurocytology·R S Cohen, V Kriho
May 1, 1987·Calcified Tissue International·T Sasaki, P R Garant
Nov 23, 1981·Brain Research·R L BeachC W Cotman
Mar 1, 1992·Developmental Biology·G E Kovalick, K Beckingham
Oct 14, 1986·European Journal of Pharmacology·B H Schmidt, J E Schultz
Dec 1, 1981·Journal of Steroid Biochemistry·A R Means, J G Chafouleas
Jun 1, 1985·Brain Research·E Fifková
Jun 1, 1990·Brain Research. Developmental Brain Research·M CiminoB Weiss
Jul 1, 1989·Brain Research. Molecular Brain Research·Z KaprielianD M Fambrough
Jan 1, 1983·Progress in Neurobiology·J J McArdle
Sep 1, 1980·Molecular and Cellular Endocrinology·A R Means, J R Dedman
Nov 1, 1983·Neuroscience·N Halász, G M Shepherd
Sep 25, 2002·Life Sciences·Arpad PalfiKaroly Gulya
May 2, 2001·The International Journal of Biochemistry & Cell Biology·C SolàJ Serratosa
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M VolpiM B Feinstein
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H SmilowitzM B Feinstein
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P Siekevitz
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P R GirardJ F Kuo
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R L KincaidM L Billingsley
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·S PatelC W Taylor

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